ArticleAnalytical chemistry2012
Nanoparticle PEBBLE sensors for quantitative nanomolar imaging of intracellular free calcium ions.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 69 citations in OpenAlex.
- Application of gold nanoclusters in fluorescence sensing and biological detection.Analytical and bioanalytical chemistry · 2024Review
- Local Environments Created by the Ligand Coating of Nanoparticles and Their Implications for Sensing and Surface Reactions.Accounts of chemical research · 2023Article
- Fluorescent nano- and microparticles for sensing cellular microenvironment: past, present and future applications.Nanoscale advances · 2023Review
- Nanoparticles for Interrogation of Cell Signaling.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023Review
- Protamine/heparin optical nanosensors based on solvatochromism.Chemical science · 2021Article
- Optimizing Calcium Detection Methods in Animal Systems: A Sandbox for Synthetic Biology.Biomolecules · 2021Review
- New generation of bioreactors that advance extracellular matrix modelling and tissue engineering.Biotechnology letters · 2019Review
- Imaging Sodium Flux during Action Potentials in Neurons with Fluorescent Nanosensors and Transparent Microelectrodes.ACS sensors · 2018Article
- A method for estimating intracellular ion concentration using optical nanosensors and ratiometric imaging.Scientific reports · 2017Article
- Ion-Selective Nanosensor for Photoacoustic and Fluorescence Imaging of Potassium.Analytical chemistry · 2017Article
- Long-wavelength analyte-sensitive luminescent probes and optical (bio)sensors.Methods and applications in fluorescence · 2015Article
- Development of an Optical Nanosensor Incorporating a pH-Sensitive Quencher Dye for Potassium Imaging.Analytical chemistry · 2015Article
- Article
- Unique diagnostic and therapeutic roles of porphyrins and phthalocyanines in photodynamic therapy, imaging and theranostics.Theranostics · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Ca(2+) is a universal second messenger and plays a major role in intracellular signaling, metabolism, and a wide range of cellular processes. To date, one of the most successful approaches for intracellular Ca(2+) measurement involves the introduction of optically sensitive Ca(2+) indicators into living cells, combined with digital imaging microscopy. However, the use of free Ca(2+) indicators for intracellular sensing and imaging has several limitations, such as nonratiometric measurement for the most-sensitive indicators, cytotoxicity of the indicators, interference from nonspecific binding caused by cellular biomacromolecules, challenging calibration, and unwanted sequestration of the indicator molecules. These problems are minimized when the Ca(2+) indicators are encapsulated inside porous and inert polyacrylamide nanoparticles. We present PEBBLE nanosensors encapsulated with rhodamine-based Ca(2+) fluorescence indicators. The rhod-2-containing PEBBLEs presented here show a stable sensing range at near-neutral pH (pH 6-9). Because of the protection of the PEBBLE matrix, the interference of protein-nonspecific binding to the indicator is minimal. The rhod-2 PEBBLEs give a nanomolar dynamic sensing range for both in-solution (K(d) = 478 nM) and intracellular (K(d) = 293 nM) measurements. These nanosensors are useful quantitative tools for the measurement and imaging of the cytosolic nanomolar free Ca(2+) levels.
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Registered trials
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