ArticleBiotechnology journal2012
Biochemical and mechanical extracellular matrix properties dictate mammary epithelial cell motility and assembly.
Article in Biotechnology journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Epithelial Cell Mechanoresponse to Matrix Viscoelasticity and Confinement Within Micropatterned Viscoelastic Hydrogels.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Zymogen granule protein 16B (ZG16B) is a druggable epigenetic target to modulate the mammary extracellular matrix.Cancer science · 2025Article
- Tissue-dependent mechanosensing by cells derived from human tumors.npj biological physics and mechanics · 2025Article
- Measuring (biological) materials mechanics with atomic force microscopy. 5. Traction force microscopy (cell traction forces).Microscopy research and technique · 2023Review
- Not all (cells) who wander are lost: Upstream migration as a pervasive mode of amoeboid cell motility.Frontiers in cell and developmental biology · 2023Review
- Laser cavitation rheology for measurement of elastic moduli and failure strain within hydrogels.Scientific reports · 2020Article
- Microstructural densification and alignment by aspiration-ejection influence cancer cell interactions with three-dimensional collagen networks.Biotechnology and bioengineering · 2020Article
- Stiffness Sensing by Cells.Physiological reviews · 2020Review
- Modulating Tumor Cell Functions by Tunable Nanopatterned Ligand Presentation.Nanomaterials (Basel, Switzerland) · 2020Review
- CD4Frontiers in immunology · 2020Article
- Effect of substrate stiffness on hepatocyte migration and cellular Young's modulus.Journal of cellular physiology · 2018Article
- Nanoscale physicochemical properties of chain- and step-growth polymerized PEG hydrogels affect cell-material interactions.Journal of biomedical materials research. Part A · 2017Article
- Dynamics of cancerous tissue correlates with invasiveness.Scientific reports · 2017Article
- Vascular smooth muscle cell durotaxis depends on extracellular matrix composition.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Force engages vinculin and promotes tumor progression by enhancing PI3K activation of phosphatidylinositol (3,4,5)-triphosphate.Cancer research · 2014Article
- Multiwell stiffness assay for the study of cell responsiveness to cytotoxic drugs.Biotechnology and bioengineering · 2014Article
- Bidirectional coupling between integrin-mediated signaling and actomyosin mechanics explains matrix-dependent intermittency of leading-edge motility.Molecular biology of the cell · 2013Article
- Tuning three-dimensional collagen matrix stiffness independently of collagen concentration modulates endothelial cell behavior.Acta biomaterialia · 2013Article
- A computational model for collective cellular motion in three dimensions: general framework and case study for cell pair dynamics.PloS one · 2013Article
- Development of polydimethylsiloxane substrates with tunable elastic modulus to study cell mechanobiology in muscle and nerve.PloS one · 2012Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Biochemical and mechanical cues of the extracellular matrix have been shown to play important roles in cell-matrix and cell-cell interactions. We have experimentally tested the combined influence of these cues to better understand cell motility, force generation, cell-cell interaction, and assembly in an in vitro breast cancer model. MCF-10A non-tumorigenic mammary epithelial cells were observed on surfaces with varying fibronectin ligand concentration and polyacrylamide gel rigidity. Our data show that cell velocity is biphasic in both matrix rigidity and adhesiveness. The maximum cell migration velocity occurs only at specific combination of substrate stiffness and ligand density. We found cell-cell interactions reduce migration velocity. However, the traction forces cells exert onto the substrate increase linearly with both cues, with cells in pairs exerting higher maximum tractions observed over single cells. A relationship between force and motility shows a maximum in single cell velocity not observed in cell pairs. Cell-cell adhesion becomes strongly favored on softer gels with elasticity ≤ 1250 Pascals (Pa), implying the existence of a compliance threshold that promotes cell-cell over cell-matrix adhesion. Finally on gels with stiffness similar to pre-malignant breast tissue, 400 Pa, cells undergo multicellular assembly and division into 3D spherical aggregates on a 2D surface.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.