ArticleBiochemical and biophysical research communications2012
The microtubule-associated protein EB1 maintains cell polarity through activation of protein kinase C.
Article in Biochemical and biophysical research communications, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 20 citations in OpenAlex.
- Comparative transcriptome analysis of endemic and epidemic Kaposi's sarcoma (KS) lesions and the secondary role of HIV-1 in KS pathogenesis.PLoS pathogens · 2020Article
- Article
- Pairing of homologous chromosomes in C. elegans meiosis requires DEB-1 - an orthologue of mammalian vinculin.Nucleus (Austin, Tex.) · 2019Article
- Dynamic microtubules drive fibroblast spreading.Biology open · 2018Article
- EB1 and cytoplasmic dynein mediate protrusion dynamics for efficient 3-dimensional cell migration.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Ras GAP-related and C-terminal domain-dependent localization and tumorigenic activities of IQGAP1 in melanoma cells.PloS one · 2017Article
- Regulation of microtubule (MT)-based cytoskeleton in the seminiferous epithelium during spermatogenesis.Seminars in cell & developmental biology · 2016Review
- GAR22β regulates cell migration, sperm motility, and axoneme structure.Molecular biology of the cell · 2016Article
- A direct interaction between fascin and microtubules contributes to adhesion dynamics and cell migration.Journal of cell science · 2015Article
- EB1 regulates tubulin and actin cytoskeletal networks at the sertoli cell blood-testis barrier in male rats: an in vitro study.Endocrinology · 2015Article
- The microtubule plus-end tracking proteins SPR1 and EB1b interact to maintain polar cell elongation and directional organ growth in Arabidopsis.The Plant cell · 2014Article
- Physical biology in cancer. 5. The rocky road of metastasis: the role of cytoskeletal mechanics in cell migratory response to 3D matrix topography.American journal of physiology. Cell physiology · 2014Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The plus-ends of microtubules target the cell cortex to modulate actin protrusion dynamics and polarity, but little is known of the molecular mechanism that couples the interaction. EB1 protein associates with the plus-ends of microtubules, placing EB1 in an ideal spatial position to mediate microtubule-actin cross talk. The objective of the current study was to further understand intracellular signaling involved in EB1-dependent cell polarity and motility. B16F10 mouse melanoma cells were depleted of EB1 protein using short hair-pin RNA interference. Correlative live cell-immunofluorescence microscopy was performed to determine localization of WAVE2 and IQGAP1 to protruding versus retracting edges. EB1 knock down caused poor subcellular separation of WAVE2 and IQGAP1, and overall decreased localization. Activation of PKC corrected defects in WAVE2 and IQGAP1 localization, cell spreading and cell shape to levels observed in control cells, but did not correct defects in cell migration. Consistent with these findings, decreased PKC phosphorylation was observed in EB1 knock down cells. These findings support a model where EB1 protein links microtubules to actin protrusion and cell polarity through signaling pathways involving PKC.
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