ArticleMethods (San Diego, Calif.)2012
Purification of cardiac cells from Drosophila embryos.
Article in Methods (San Diego, Calif.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 13 citations in OpenAlex.
- FGF signaling directs myotube guidance by regulating Rac activity.Development (Cambridge, England) · 2020Article
- Purification of Low-abundant Cells in the Drosophila Visual System.Journal of visualized experiments : JoVE · 2018Article
- Genome-Wide Approaches to Drosophila Heart Development.Journal of cardiovascular development and disease · 2016Article
- On the Morphology of the Drosophila Heart.Journal of cardiovascular development and disease · 2016Review
- The Drosophila surface glia transcriptome: evolutionary conserved blood-brain barrier processes.Frontiers in neuroscience · 2014Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Recent genome-level innovations have enabled the identification of the entire cadre of genes that are expressed in specific tissues at particular developmental times. However, to be informative as to how individual cell types develop, this process relies upon the successful and efficient purification of cells for a particular tissue. Here, we describe a method to isolate cardiac cells from Drosophila embryos. We generated transgenic embryos in which a cardiac-specific enhancer of the Sulphonylurea receptor (Sur) gene drove expression of the green fluorescent protein (GFP) gene. Homogenized embryos were subjected to fluorescence activated cell sorting (FACS), resulting in approximately 50,000 cardiac cells purified. The prevalence of cardiac cells in the purified population was high, based upon a significant enrichment for cardiac-specific marker genes, including Sur and Toll. This enrichment also enabled the identification of cardiac-specific alternatively spliced isoforms of the Zasp66 gene. In the future, this approach can be used to describe the cardiac transcriptome of Drosophila at distinct stages of embryonic development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.