ArticleTraffic (Copenhagen, Denmark)2012
Multiple biosynthetic trafficking routes for apically secreted proteins in MDCK cells.
Article in Traffic (Copenhagen, Denmark), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Newly synthesized and recycling pools of the apical protein gp135 do not occupy the same compartments.Traffic (Copenhagen, Denmark) · 2016Article
- Complex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment.The Journal of biological chemistry · 2014Article
- Global ablation of the mouse Rab11a gene impairs early embryogenesis and matrix metalloproteinase secretion.The Journal of biological chemistry · 2014Article
- Galectin-4-mediated transcytosis of transferrin receptor.Journal of cell science · 2014Article
- Myosin Vb mediated plasma membrane homeostasis regulates peridermal cell size and maintains tissue homeostasis in the zebrafish epidermis.PLoS genetics · 2014Article
- Trafficking to the apical and basolateral membranes in polarized epithelial cells.Journal of the American Society of Nephrology : JASN · 2014Review
- Rab11a-positive compartments in proximal tubule cells sort fluid-phase and membrane cargo.American journal of physiology. Cell physiology · 2014Article
- Three basic residues of intracellular loop 3 of the beta-1 adrenergic receptor are required for golgin-160-dependent trafficking.International journal of molecular sciences · 2014Article
- VAMP7 modulates ciliary biogenesis in kidney cells.PloS one · 2014Article
- Membrane trafficking in podocyte health and disease.Pediatric nephrology (Berlin, Germany) · 2013Review
- Multiple motifs regulate apical sorting of p75 via a mechanism that involves dimerization and higher-order oligomerization.Molecular biology of the cell · 2013Article
- Role of membrane traffic in the generation of epithelial cell asymmetry.Nature cell biology · 2012Review
- Sialylation of N-linked glycans mediates apical delivery of endolyn in MDCK cells via a galectin-9-dependent mechanism.Molecular biology of the cell · 2012Article
- Polarized sorting and trafficking in epithelial cells.Cell research · 2012Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Many newly synthesized membrane proteins traverse endocytic intermediates en route to the surface in polarized epithelial cells; however, the biosynthetic itinerary of secreted proteins has not been elucidated. We monitored the trafficking route of two secreted proteins with different apical sorting signals: the N-glycan-dependent cargo glycosylated growth hormone (gGH) and Ensol, a soluble version of endolyn whose apical sorting is independent of N-glycans. Both proteins were observed to colocalize in part with apical recycling endosome (ARE) markers. Cargo that lacks an apical targeting signal and is secreted in a nonpolarized manner did not localize to the ARE. Expression of a dominant-negative mutant of myosin Vb, which disrupts ARE export of glycan-dependent membrane proteins, selectively inhibited apical release of gGH but not Ensol. Fluorescence recovery after photobleaching (FRAP) measurements revealed that gGH in the ARE was less mobile than Ensol, consistent with tethering to a sorting receptor. However, knockdown of galectin-3 or galectin-4, lectins implicated in apical sorting, had no effect on the rate or polarity of gGH secretion. Together, our results suggest that apically secreted cargoes selectively access the ARE and are exported via differentially regulated pathways.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.