ArticleBMC genomics2011
Human native lipoprotein-induced de novo DNA methylation is associated with repression of inflammatory genes in THP-1 macrophages.
Article in BMC genomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 41 citations in OpenAlex.
- The functional significance of vascular DNA hypermethylation in atherosclerosis: a historical perspective.Frontiers in pharmacology · 2025Review
- LDL-Dependent Regulation of TNFα/PGEInflammation · 2023Article
- Article
- Epigenetic regulation of inflammatory factors in adipose tissue.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2021Review
- DNA methylation microarrays identify epigenetically regulated lipid related genes in obese patients with hypercholesterolemia.Molecular medicine (Cambridge, Mass.) · 2020Article
- Is Any Cardiovascular Disease-Specific DNA Methylation Biomarker Within Reach?Current atherosclerosis reports · 2020Review
- Aberrantly Methylated-Differentially Expressed Genes Identify Novel Atherosclerosis Risk Subtypes.Frontiers in genetics · 2020Article
- Nutritional Factors Modulating Alu Methylation in an Italian Sample from The Mark-Age Study Including Offspring of Healthy Nonagenarians.Nutrients · 2019Article
- Associations between atherosclerosis and neurological diseases, beyond ischemia-induced cerebral damage.Reviews in endocrine & metabolic disorders · 2019Review
- Relationship between Long Interspersed Nuclear Element-1 DNA Methylation in Leukocytes and Dyslipidemia in the Japanese General Population.Journal of atherosclerosis and thrombosis · 2018Article
- Folic acid modulates VPO1 DNA methylation levels and alleviates oxidative stress-induced apoptosis in vivo and in vitro.Redox biology · 2018Article
- Liver X Receptor-Binding DNA Motif Associated With Atherosclerosis-Specific DNA Methylation Profiles ofJournal of the American Heart Association · 2018Article
- The effects of dietary polyunsaturated fatty acids on miR-126 promoter DNA methylation status and VEGF protein expression in the colorectal cancer cells.Genes & nutrition · 2018Article
- DNA Methylation in Stroke. Update of Latest Advances.Computational and structural biotechnology journal · 2018Review
- Connecting the Dots Between Fatty Acids, Mitochondrial Function, and DNA Methylation in Atherosclerosis.Current atherosclerosis reports · 2017Review
- Associations between whole peripheral blood fatty acids and DNA methylation in humans.Scientific reports · 2016Article
- Arachidonic and oleic acid exert distinct effects on the DNA methylome.Epigenetics · 2016Article
- The trans fatty acid elaidate affects the global DNA methylation profile of cultured cells and in vivo.Lipids in health and disease · 2016Article
- Profiling post-translational modifications of histones in human monocyte-derived macrophages.Proteome science · 2015Article
- Epipolymorphisms within lipoprotein genes contribute independently to plasma lipid levels in familial hypercholesterolemia.Epigenetics · 2014Article
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Authors and funding
14 authors at 7 institutions in 5 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundWe previously showed that a VLDL- and LDL-rich mix of human native lipoproteins induces a set of repressive epigenetic marks, i.e. de novo DNA methylation, histone 4 hypoacetylation and histone 4 lysine 20 (H4K20) hypermethylation in THP-1 macrophages. Here, we: 1) ask what gene expression changes accompany these epigenetic responses; 2) test the involvement of candidate factors mediating the latter. We exploited genome expression arrays to identify target genes for lipoprotein-induced silencing, in addition to RNAi and expression studies to test the involvement of candidate mediating factors. The study was conducted in human THP-1 macrophages.
resultsNative lipoprotein-induced de novo DNA methylation was associated with a general repression of various critical genes for macrophage function, including pro-inflammatory genes. Lipoproteins showed differential effects on epigenetic marks, as de novo DNA methylation was induced by VLDL and to a lesser extent by LDL, but not by HDL, and VLDL induced H4K20 hypermethylation, while HDL caused H4 deacetylation. The analysis of candidate factors mediating VLDL-induced DNA hypermethylation revealed that this response was: 1) surprisingly, mediated exclusively by the canonical maintenance DNA methyltransferase DNMT1, and 2) independent of the Dicer/micro-RNA pathway.
conclusionsOur work provides novel insights into epigenetic gene regulation by native lipoproteins. Furthermore, we provide an example of DNMT1 acting as a de novo DNA methyltransferase independently of canonical de novo enzymes, and show proof of principle that de novo DNA methylation can occur independently of a functional Dicer/micro-RNA pathway in mammals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.