ArticleBMC veterinary research2011
Identification of Mycobacterium spp. of veterinary importance using rpoB gene sequencing.
Article in BMC veterinary research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Coinfection byJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2024Article
- A Rare Case of Osteomyelitis of an Ankle Caused byAntibiotics (Basel, Switzerland) · 2023Article
- Short SignatureMicrobiology spectrum · 2022Article
- Pathology and Prevalence of Antibiotic-Resistant Bacteria: A Study of 398 Pet Reptiles.Animals : an open access journal from MDPI · 2022Article
- High female mortality caused by an atypicalThe Canadian veterinary journal = La revue veterinaire canadienne · 2022Article
- Use of slide scrape lysates for polymerase chain reaction confirmation of disseminatedThe Canadian veterinary journal = La revue veterinaire canadienne · 2019Article
- Molecular identification of Mycobacterium avium subsp. silvaticum by duplex high-resolution melt analysis and subspecies-specific real-time PCR.Journal of clinical microbiology · 2015Article
- Utility of rpoB gene sequencing for identification of nontuberculous mycobacteria in the Netherlands.Journal of clinical microbiology · 2014Article
- Draft Genome Sequence of a Mycobacterium avium Complex Isolate from a Broadbill Bird.Genome announcements · 2014Article
- First case of pulmonary disease caused by a Mycobacterium avium complex strain of presumed veterinary origin in an adult human patient.Journal of clinical microbiology · 2013Article
- Isolation of mycobacteria from clinical samples collected in the United States from 2004 to 2011.BMC veterinary research · 2013Article
- Article
- Field application of serodiagnostics to identify elephants with tuberculosis prior to case confirmation by culture.Clinical and vaccine immunology : CVI · 2012Article
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Authors and funding
6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundStudies conducted on Mycobacterium spp. isolated from human patients indicate that sequencing of a 711 bp portion of the rpoB gene can be useful in assigning a species identity, particularly for members of the Mycobacterium avium complex (MAC). Given that MAC are important pathogens in livestock, companion animals, and zoo/exotic animals, we were interested in evaluating the use of rpoB sequencing for identification of Mycobacterium isolates of veterinary origin.
resultsA total of 386 isolates, collected over 2008 - June 2011 from 378 animals (amphibians, reptiles, birds, and mammals) underwent PCR and sequencing of a ~ 711 bp portion of the rpoB gene; 310 isolates (80%) were identified to the species level based on similarity at ≥ 98% with a reference sequence. The remaining 76 isolates (20%) displayed < 98% similarity with reference sequences and were assigned to a clade based on their location in a neighbor-joining tree containing reference sequences. For a subset of 236 isolates that received both 16S rRNA and rpoB sequencing, 167 (70%) displayed a similar species/clade assignation for both sequencing methods. For the remaining 69 isolates, species/clade identities were different with each sequencing method. Mycobacterium avium subsp. hominissuis was the species most frequently isolated from specimens from pigs, cervids, companion animals, cattle, and exotic/zoo animals.
conclusionsrpoB sequencing proved useful in identifying Mycobacterium isolates of veterinary origin to clade, species, or subspecies levels, particularly for assemblages (such as the MAC) where 16S rRNA sequencing alone is not adequate to demarcate these taxa. rpoB sequencing can represent a cost-effective identification tool suitable for routine use in the veterinary diagnostic laboratory.
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