ArticleThe Journal of cell biology1990
The expression of myosin genes in developing skeletal muscle in the mouse embryo.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 87 papers.
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Who cites it
87 citing papers in PubMed, 268 citations in OpenAlex.
- Transcriptomic analysis of skeletal muscle regeneration across mouse lifespan identifies altered stem cell states.Nature aging · 2024Article
- Musculoskeletal defects associated with myosin heavy chain-embryonic loss of function are mediated by the YAP signaling pathway.EMBO molecular medicine · 2023Article
- Molecular Mechanism ofGenes · 2023Article
- The emergence of the stem cell niche.Trends in cell biology · 2023Review
- The Emergence of Embryonic Myosin Heavy Chain during Branchiomeric Muscle Development.Life (Basel, Switzerland) · 2022Article
- Differences in gene expression between the primary and secondary inferior oblique overaction.Translational pediatrics · 2022Article
- KMT5B is required for early motor development.Frontiers in genetics · 2022Article
- Reorganization of chromatin architecture during prenatal development of porcine skeletal muscle.DNA research : an international journal for rapid publication of reports on genes and genomes · 2021Article
- Insights into myosin regulatory and essential light chains: a focus on their roles in cardiac and skeletal muscle function, development and disease.Journal of muscle research and cell motility · 2020Article
- Single-Cell Analysis of the Muscle Stem Cell Hierarchy Identifies Heterotypic Communication Signals Involved in Skeletal Muscle Regeneration.Cell reports · 2020Article
- Myopathy reversion in mice after restauration of mitochondrial complex I.EMBO molecular medicine · 2020Article
- NDUFAB1 protects against obesity and insulin resistance by enhancing mitochondrial metabolism.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Dynamics of Non-Canonical Amino Acid-Labeled Intra- and Extracellular Proteins in the Developing Mouse.Cellular and molecular bioengineering · 2019Article
- Muscle ciliary neurotrophic factor receptor α contributes to motor neuron STAT3 activation following peripheral nerve lesion.The European journal of neuroscience · 2019Article
- Kinin-B2 Receptor Activity in Skeletal Muscle Regeneration and Myoblast Differentiation.Stem cell reviews and reports · 2019Article
- Post-transcriptional regulation of Pabpn1 by the RNA binding protein HuR.Nucleic acids research · 2018Article
- ATAD3 controls mitochondrial cristae structure in mouse muscle, influencing mtDNA replication and cholesterol levels.Journal of cell science · 2018Article
- Thick Filament Protein Network, Functions, and Disease Association.Comprehensive Physiology · 2018Review
- Expression profiling of disease progression in canine model of Duchenne muscular dystrophy.PloS one · 2018Article
- RhoA/ROCK inhibition improves the beneficial effects of glucocorticoid treatment in dystrophic muscle: implications for stem cell depletion.Human molecular genetics · 2017Article
27 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using in situ hybridization, we have investigated the temporal sequence of myosin gene expression in the developing skeletal muscle masses of mouse embryos. The probes used were isoform-specific, 35S-labeled antisense cRNAs to the known sarcomeric myosin heavy chain and myosin alkali light chain gene transcripts. Results showed that both cardiac and skeletal myosin heavy chain and myosin light chain mRNAs were first detected between 9 and 10 d post coitum (p.c.) in the myotomes of the most rostral somites. Myosin transcripts appeared in more caudal somites at later stages in a developmental gradient. The earliest myosin heavy chain transcripts detected code for the embryonic skeletal (MHCemb) and beta-cardiac (MHC beta) isoforms. Perinatal myosin heavy chain (MHCpn) transcripts begin to accumulate at 10.5 d p.c., which is much earlier than previously reported. At this stage, MHCemb is the major MHC transcript. By 12.5 d p.c., MHCpn and MHCemb mRNAs are present to an equal extent, and by 15.5 d p.c. the MHCpn transcript is the major MHC mRNA detected. Cardiac MHC beta transcripts are always present as a minor component. In contrast, the cardiac MLC1A mRNA is initially more abundant than that encoding the skeletal MLC1F isoform. By 12.5 d p.c. the two MLC mRNAs are present at similar levels, and by 15.5 d p.c., MLC1F is the predominant MLC transcript detected. Transcripts for the ventricular/slow (MLC1V) and another fast skeletal myosin light chain (MLC3F) are not detected in skeletal muscle before 15 d p.c., which marks the beginning of the fetal stage of muscle development. This is the first stage at which we can detect differences in expression of myosin genes between developing muscle fibers. We conclude that, during the development of the myotome and body wall muscles, different myosin genes follow independent patterns of activation and accumulation. The data presented are the first detailed study of myosin gene expression at these early stages of skeletal muscle development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.