ArticleThe Journal of cell biology1990
The SPA2 gene of Saccharomyces cerevisiae is important for pheromone-induced morphogenesis and efficient mating.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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Who cites it
78 citing papers in PubMed, 151 citations in OpenAlex.
- Polarisome core component FgPea2 regulates FgBoi2-mediated polarized growth, pathogenicity and environmental stress in Fusarium graminearum.Stress biology · 2026Article
- Oligomerization and exocyst coupling underlie Spa2-mediated focusing of polarized growth in fission yeast.Journal of cell science · 2025Article
- A positive feedback loop involving the Spa2 SHD domain contributes to focal polarization.PloS one · 2022Article
- Type V myosin focuses the polarisome and shapes the tip of yeast cells.The Journal of cell biology · 2021Article
- Polarisome scaffolder Spa2-mediated macromolecular condensation of Aip5 for actin polymerization.Nature communications · 2019Article
- Cell polarity protein Spa2 coordinates Chs2 incorporation at the division site in budding yeast.PLoS genetics · 2018Article
- The Cdc15 and Imp2 SH3 domains cooperatively scaffold a network of proteins that redundantly ensure efficient cell division in fission yeast.Molecular biology of the cell · 2015Article
- A spindle pole antigen gene MoSPA2 is important for polar cell growth of vegetative hyphae and conidia, but is dispensable for pathogenicity in Magnaporthe oryzae.Current genetics · 2014Article
- Spatial segregation of polarity factors into distinct cortical clusters is required for cell polarity control.Nature communications · 2013Article
- The yeast AAA+ chaperone Hsp104 is part of a network that links the actin cytoskeleton with the inheritance of damaged proteins.Molecular and cellular biology · 2009Article
- Systematic definition of protein constituents along the major polarization axis reveals an adaptive reuse of the polarization machinery in pheromone-treated budding yeast.Journal of proteome research · 2009Article
- Dissecting colony development of Neurospora crassa using mRNA profiling and comparative genomics approaches.Eukaryotic cell · 2008Article
- Dynamic localization of yeast Fus2p to an expanding ring at the cell fusion junction during mating.The Journal of cell biology · 2008Article
- Functional characterization of Aspergillus nidulans homologues of Saccharomyces cerevisiae Spa2 and Bud6.Eukaryotic cell · 2006Article
- Cell polarity protein Spa2P associates with proteins involved in actin function in Saccharomyces cerevisiae.Molecular biology of the cell · 2005Article
- Polarized morphogenesis regulator Spa2 is required for the function of putative stretch-activated Ca2+-permeable channel component Mid1 in Saccharomyces cerevisiae.Eukaryotic cell · 2005Article
- PAK kinases Ste20 and Pak1 govern cell polarity at different stages of mating in Cryptococcus neoformans.Molecular biology of the cell · 2004Article
- Lrg1p Is a Rho1 GTPase-activating protein required for efficient cell fusion in yeast.Genetics · 2004Article
- Pheromone-induced polarization is dependent on the Fus3p MAPK acting through the formin Bni1p.The Journal of cell biology · 2004Article
- Regulation of polarized growth initiation and termination cycles by the polarisome and Cdc42 regulators.The Journal of cell biology · 2004Article
18 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Upon exposure to mating pheromone, Saccharomyces cerevisiae undergoes cellular differentiation to form a morphologically distinct cell called a "shmoo". Double staining experiments revealed that both the SPA2 protein and actin localize to the shmoo tip which is the site of polarized cell growth. Actin concentrates as spots throughout the shmoo projection, while SPA2 localizes as a sharp patch at the shmoo tip. DNA sequence analysis of the SPA2 gene revealed an open reading frame 1,466 codons in length; the predicted protein sequence contains many internal repeats including a nine amino acid sequence that is imperfectly repeated 25 times. Portions of the SPA2 sequence exhibit a low-level similarity to proteins containing coiled-coil structures. Yeast cells containing a large deletion of the SPA2 gene are similar in growth rate to wild-type cells. However, spa2 mutant cells are impaired in their ability to form shmoos upon exposure to mating pheromone, and they do not mate efficiently with other spa2 mutant cells. Thus, we suggest that the SPA2 protein plays a critical role in cellular morphogenesis during mating, perhaps as a cytoskeletal protein.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.