ArticleAnalytical chemistry2012
In vivo neurochemical monitoring using benzoyl chloride derivatization and liquid chromatography-mass spectrometry.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers, 1 of them a synthesis that pooled it.
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Who cites it
96 citing papers in PubMed, 1 synthesis or guideline pooled it, 242 citations in OpenAlex.
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- Medial prefrontal cortical neurotransmitters reactive to relapse-promoting and relapse-suppressing cues in male rats trained to self-administer cocaine or alcohol.Addiction neuroscience · 2026Article
- GABA: The Peacekeeper Neurotransmitter-Gut-microbiota Derived Origins and Salivary Biomarker Detection Using Elisa.Annals of neurosciences · 2025Review
- Sampling Probes Microfabricated from Parylene‑C for In Vivo Neurochemical Monitoring.ACS measurement science au · 2025Article
- Controlled electrodeposition of tungsten oxide nanoparticles on MXene nanosheets-framework for ultrasensitive light enhanced detection of dopamine.Scientific reports · 2025Article
- Timescales of dopamine release in the striatum as a window into hierarchical control.Current opinion in behavioral sciences · 2025Article
- Detection of Neuronal Glutamate in Brain Extracellular Space In Vivo Using Microdialysis and Metabolic Labeling with Glutamine.ACS chemical neuroscience · 2025Article
- Modulation of copper-induced neurotoxicity by monoisoamyl 2,3-dimercaptosuccinic acid loaded nanoparticles through inhibition of mitophagy and reduction of oxidative stress in SH-SY5Y cells.Toxicology reports · 2025Article
- Determination of Thyroid Hormones and 11 Metabolites in the Human Serum Using a Simple Derivatization Strategy and Analysis by Isotope-Dilution Liquid Chromatography Tandem Mass Spectrometry.Analytical chemistry · 2025Article
- Production of Acetylcholine by Podocytes and its Protection from Kidney Injury in GN.Journal of the American Society of Nephrology : JASN · 2025Article
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- Dysregulated acetylcholine-mediated dopamine neurotransmission in the eIF4E Tg mouse model of autism spectrum disorders.Cell reports · 2024Article
- Wideband ratiometric measurement of tonic and phasic dopamine release in the striatum.bioRxiv : the preprint server for biology · 2024Article
- In-Depth Chemical Analysis of the Brain Extracellular Space UsingAnalytical chemistry · 2024Article
- Deep learning prediction of electrospray ionization tandem mass spectra of chemically derived molecules.Nature communications · 2024Article
- Systematic evaluation of benzoylation for liquid chromatography-mass spectrometry analysis of different analyte classes.Journal of chromatography. A · 2024Article
36 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In vivo neurochemical monitoring using microdialysis sampling is important in neuroscience because it allows correlation of neurotransmission with behavior, disease state, and drug concentrations in the intact brain. A significant limitation of current practice is that different assays are utilized for measuring each class of neurotransmitter. We present a high performance liquid chromatography (HPLC)-tandem mass spectrometry method that utilizes benzoyl chloride for determination of the most common low molecular weight neurotransmitters and metabolites. In this method, 17 analytes were separated in 8 min. The limit of detection was 0.03-0.2 nM for monoamine neurotransmitters, 0.05-11 nM for monoamine metabolites, 2-250 nM for amino acids, 0.5 nM for acetylcholine, 2 nM for histamine, and 25 nM for adenosine at sample volume of 5 μL. Relative standard deviation for repeated analysis at concentrations expected in vivo averaged 7% (n = 3). Commercially available (13)C benzoyl chloride was used to generate isotope-labeled internal standards for improved quantification. To demonstrate utility of the method for study of small brain regions, the GABA(A) receptor antagonist bicuculline (50 μM) was infused into a rat ventral tegmental area while recording neurotransmitter concentration locally and in nucleus accumbens, revealing complex GABAergic control over mesolimbic processes. To demonstrate high temporal resolution monitoring, samples were collected every 60 s while neostigmine, an acetylcholine esterase inhibitor, was infused into the medial prefrontal cortex. This experiment revealed selective positive control of acetylcholine over cortical glutamate.
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