ArticleThe Journal of biological chemistry2012
Integrins regulate microtubule nucleating activity of centrosome through mitogen-activated protein kinase/extracellular signal-regulated kinase kinase/extracellular signal-regulated kinase (MEK/ERK) signaling.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 41 citations in OpenAlex.
- Centrosome-Signaling Pathway Crosstalk: A Core Hub From Cellular Homeostasis to Disease.Cytoskeleton (Hoboken, N.J.) · 2026Review
- Inhibitory effects of patchouli alcohol on hepatocellular carcinoma growth through accumulation of oxidative stress and inactivation of androgen receptor signaling.International journal of medical sciences · 2026Article
- Dysregulation of integrin αvβ3 and α5β1 impedes migration of placental endothelial cells in fetal growth restriction.Development (Cambridge, England) · 2022Article
- Article
- Microtubule Acetylation Controls MDA-MB-231 Breast Cancer Cell Invasion through the Modulation of Endoplasmic Reticulum Stress.International journal of molecular sciences · 2021Article
- Fibronectin-integrin signaling regulates PLVAP localization at endothelial fenestrae by microtubule stabilization.Cell and tissue research · 2021Article
- DNA damage promotes microtubule dynamics through a DNA-PK-AKT axis for enhanced repair.The Journal of cell biology · 2021Article
- Antagonism betweeneLife · 2021Article
- LUZP1 and the tumor suppressor EPLIN modulate actin stability to restrict primary cilia formation.The Journal of cell biology · 2020Article
- Crosstalk Between Pheromone Signaling and NADPH Oxidase Complexes Coordinates Fungal Developmental Processes.Frontiers in microbiology · 2020Article
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- Integrin and microtubule crosstalk in the regulation of cellular processes.Cellular and molecular life sciences : CMLS · 2018Review
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- A parental requirement for dual-specificity phosphatase 6 in zebrafish.BMC developmental biology · 2018Article
- microRNA-219-5p inhibits epithelial-mesenchymal transition and metastasis of colorectal cancer by targeting lymphoid enhancer-binding factor 1.Cancer science · 2017Article
- HSP70 regulates the function of mitotic centrosomes.Cellular and molecular life sciences : CMLS · 2016Article
- Stage-specific control of stem cell niche architecture in the Drosophila testis by the posterior Hox gene Abd-B.Computational and structural biotechnology journal · 2015Review
- Rationale and preclinical efficacy of a novel anti-EMP2 antibody for the treatment of invasive breast cancer.Molecular cancer therapeutics · 2014Article
- Integrins promote cytokinesis through the RSK signaling axis.Journal of cell science · 2014Article
- Glioma cell proliferation controlled by ERK activity-dependent surface expression of PDGFRA.PloS one · 2014Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Microtubule nucleation is an essential step in the formation of the microtubule cytoskeleton. We recently showed that androgen and Src promote microtubule nucleation and γ-tubulin accumulation at the centrosome. Here, we explore the mechanisms by which androgen and Src regulate these processes and ask whether integrins play a role. We perturb integrin function by a tyrosine-to-alanine substitution in membrane-proximal NPIY motif in the integrin β1 tail and show that this mutant substantially decreases microtubule nucleation and γ-tubulin accumulation at the centrosome. Because androgen stimulation promotes the interaction of the androgen receptor with Src, resulting in PI3K/AKT and MEK/ERK signaling, we asked whether these pathways are inhibited by the mutant integrin and whether they regulate microtubule nucleation. Our results indicate that the formation of the androgen receptor-Src complex and the activation of downstream pathways are significantly suppressed when cells are adhered by the mutant integrin. Inhibitor studies indicate that microtubule nucleation requires MEK/ERK but not PI3K/AKT signaling. Importantly, the expression of activated RAF-1 is sufficient to rescue microtubule nucleation inhibited by the mutant integrin by promoting the centrosomal accumulation of γ-tubulin. Our data define a novel paradigm of integrin signaling, where integrins regulate microtubule nucleation by promoting the formation of androgen receptor-Src signaling complexes to activate the MEK/ERK signaling pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.