ArticleMicrobiology (Reading, England)2012
The incongruent gelatinase genotype and phenotype in Enterococcus faecalis are due to shutting off the ability to respond to the gelatinase biosynthesis-activating pheromone (GBAP) quorum-sensing signal.
Article in Microbiology (Reading, England), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 30 citations in OpenAlex.
- Biofilm Formation, Virulence Traits, and Antimicrobial Resistance Profiles ofInternational journal of microbiology · 2026Article
- Genotypic and phenotypic characterization ofbioRxiv : the preprint server for biology · 2024Article
- Enterococcal quorum-controlled protease alters phage infection.FEMS microbes · 2024Article
- The Phosphatase Bph and Peptidyl-Prolyl Isomerase PrsA Are Required for Gelatinase Expression and Activity in Enterococcus faecalis.Journal of bacteriology · 2022Article
- Review
- Fluorescent reporter plasmids for single-cell and bulk-level composition assays in E. faecalis.PloS one · 2020Article
- Identification of Collagenolytic Bacteria in Human Samples: Screening Methods and Clinical Implications for Resolving and Preventing Anastomotic Leaks and Wound Complications.Diseases of the colon and rectum · 2019Article
- The Influence of Heat-KilledFrontiers in microbiology · 2019Article
- Enterococcus faecalis Promotes Innate Immune Suppression and Polymicrobial Catheter-Associated Urinary Tract Infection.Infection and immunity · 2017Article
- Population Density Modulates Drug Inhibition and Gives Rise to Potential Bistability of Treatment Outcomes for Bacterial Infections.PLoS computational biology · 2016Article
- Comprehensive molecular, genomic and phenotypic analysis of a major clone of Enterococcus faecalis MLST ST40.BMC genomics · 2015Article
- Targeting agr- and agr-Like quorum sensing systems for development of common therapeutics to treat multiple gram-positive bacterial infections.Sensors (Basel, Switzerland) · 2013Review
- Drosophila host model reveals new enterococcus faecalis quorum-sensing associated virulence factors.PloS one · 2013Article
- The Enterococcus faecalis exoproteome: identification and temporal regulation by Fsr.PloS one · 2012Article
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Authors and funding
9 authors at 4 institutions in 4 countries.
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Abstract
The concomitant presence of a complete fsr quorum-sensing system and gelE-sprE operons in Enterococcus faecalis is known to be essential for the detection of gelatinase activity. However, there are reports of the absence of gelatinase activity despite the presence of complete fsr and gelE loci. In order to understand this incongruence between genotype and phenotype we sequenced fsr and gelE loci of the E. faecalis LN68 strain, which was previously found to carry both operons but to lack gelatinase activity. Of the 59 nucleotide differences detected compared with the gelatinase-positive V583 strain, we found a nonsense mutation (a premature STOP codon) predicted to truncate the ATPase sensor domain of the FsrC protein, responsible for sensing and transducing the signal from the quorum-sensing molecule. Strain LN68 was highly affected in the expression of the gelE and sprE genes, further supporting the lack of Fsr-dependent gelE induction. When we constructed a V583 mutant with the same premature stop mutation in the fsrC gene the resulting strain was no longer able to degrade gelatin. We conclude that the reduced ability to transduce the quorum-sensing signal of the prematurely truncated FsrC protein is sufficient to explain the negative gelatinase phenotype. As the incongruent genotype and phenotype is detected in natural isolates, we believe that the silencing of the quorum-sensing system Fsr may be beneficial for some E. faecalis strains.
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