ArticleNucleic acids research2012
An exogenous chloroplast genome for complex sequence manipulation in algae.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 65 citations in OpenAlex.
- Editing and synthetic engineering of chloroplast genomes.Plant communications · 2026Review
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Article
- Microalgal biorefineries: a systematic review of technological trade-offs and innovation pathways.Biotechnology for biofuels and bioproducts · 2025Review
- Cloning a Chloroplast Genome inBio-protocol · 2025Article
- Design and assembly of the 117-kb Phaeodactylum tricornutum chloroplast genome.Plant physiology · 2024Article
- The synthetic future of algal genomes.Cell genomics · 2024Review
- From qualitative to quantitative: the state of the art and challenges for plant synthetic biology.Quantitative biology (Beijing, China) · 2023Review
- Cross-species microbial genome transfer: a Review.Frontiers in bioengineering and biotechnology · 2023Review
- Harnessing the Algal Chloroplast for Heterologous Protein Production.Microorganisms · 2022Review
- Synthetic Genomics From a Yeast Perspective.Frontiers in bioengineering and biotechnology · 2022Review
- Biocircuits in plants and eukaryotic algae.Frontiers in plant science · 2022Review
- Nanotechnology Approaches for Chloroplast Biotechnology Advancements.Frontiers in plant science · 2021Review
- The Algal Chloroplast as a Testbed for Synthetic Biology Designs Aimed at Radically Rewiring Plant Metabolism.Frontiers in plant science · 2021Review
- An overview of anoxygenic phototrophic bacteria and their applications in environmental biotechnology for sustainable Resource recovery.Biotechnology reports (Amsterdam, Netherlands) · 2020Review
- Cloning ofBiology · 2020Article
- Plant Biosystems Design Research Roadmap 1.0.Biodesign research · 2020Review
- Reconfiguring Plant Metabolism for Biodegradable Plastic Production.Biodesign research · 2020Review
- Recent Advances and Current Challenges in Synthetic Biology of the Plastid Genetic System and Metabolism.Plant physiology · 2019Review
- Green biologics: The algal chloroplast as a platform for making biopharmaceuticals.Bioengineered · 2018Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We demonstrate a system for cloning and modifying the chloroplast genome from the green alga, Chlamydomonas reinhardtii. Through extensive use of sequence stabilization strategies, the ex vivo genome is assembled in yeast from a collection of overlapping fragments. The assembled genome is then moved into bacteria for large-scale preparations and transformed into C. reinhardtii cells. This system also allows for the generation of simultaneous, systematic and complex genetic modifications at multiple loci in vivo. We use this system to substitute genes encoding core subunits of the photosynthetic apparatus with orthologs from a related alga, Scenedesmus obliquus. Once transformed into algae, the substituted genome recombines with the endogenous genome, resulting in a hybrid plastome comprising modifications in disparate loci. The in vivo function of the genomes described herein demonstrates that simultaneous engineering of multiple sites within the chloroplast genome is now possible. This work represents the first steps toward a novel approach for creating genetic diversity in any or all regions of a chloroplast genome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.