ArticleBiochemical and biophysical research communications2011
Extracellular low pH affects circadian rhythm expression in human primary fibroblasts.
Article in Biochemical and biophysical research communications, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- Repetitive mild traumatic brain injury alters central and peripheral clock gene expression in the adolescent rat.Neurobiology of sleep and circadian rhythms · 2023Article
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- 12-h clock regulation of genetic information flow by XBP1s.PLoS biology · 2020Article
- The molecular clock in the skin, its functionality, and how it is disrupted in cutaneous melanoma: a new pharmacological target?Cellular and molecular life sciences : CMLS · 2019Review
- A Method for Culturing Mouse Whisker Follicles to Study Circadian RhythmsBio-protocol · 2019Article
- Maternal eating behavior is a major synchronizer of fetal and postnatal peripheral clocks in mice.Cellular and molecular life sciences : CMLS · 2018Article
- A simple method using ex vivo culture of hair follicle tissue to investigate intrinsic circadian characteristics in humans.Scientific reports · 2017Article
- Circadian adaptation to cell injury stresses: a crucial interplay of BMAL1 and HSF1.The journal of physiological sciences : JPS · 2016Review
- 24-hour rhythm of aquaporin-3 function in the epidermis is regulated by molecular clocks.The Journal of investigative dermatology · 2014Article
- Analysis of the molecular pathophysiology of sleep disorders relevant to a disturbed biological clock.Molecular genetics and genomics : MGG · 2013Article
- In vitro circadian period is associated with circadian/sleep preference.Scientific reports · 2013Article
- Assessment of circadian rhythms in humans: comparison of real-time fibroblast reporter imaging with plasma melatonin.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2012Article
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Authors and funding
6 authors at 3 institutions in 1 country.
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Abstract
Circadian rhythm is a fundamental biological system involved in the regulation of various physiological functions. However, little is known about a nature or function of circadian clock in human primary cells. In the present study, we have applied in vitro real time circadian rhythm monitoring to study human clock properties using primary skin fibroblasts. Among factors that affect human physiology, slightly lower extracellular pH was chosen to test its effects on circadian rhythm expression. We established human primary fibroblast cultures obtained from three healthy subjects, stably delivered a circadian reporter gene Bmal1-luciferase, and recorded circadian rhythms in the culture medium at pH 7.2 and 6.7. At pH 7.2, robust and sustained circadian rhythms were observed with average period length 24.47 ± 0.03 h. Such rhythms were also found at pH 6.7; however, period length was significantly shortened to 22.60 ± 0.20, amplitude was increased, and damping rate was decreased. The effect of exposure to low pH on the period length was reversible. The shortened period was unlikely caused by factors affecting cell viability because cell morphology and MTT assay showed no significant difference between the two conditions. In summary, our results showed that the circadian rhythm expression is affected at pH 6.7 in human primary fibroblasts without affecting cell viability.
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