ArticlePloS one2011
Uncovering the prevalence and diversity of integrating conjugative elements in actinobacteria.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 56 citations in OpenAlex.
- Landscape of mobile genetic elements and their functional cargo across the gastrointestinal tract microbiomes in ruminants.Microbiome · 2025Article
- Exploring the Accessory Genome of Multidrug-ResistantAntibiotics (Basel, Switzerland) · 2023Article
- A systematic approach to classify and characterize genomic islands driven by conjugative mobility using protein signatures.Nucleic acids research · 2023Article
- ICEscreen: a tool to detect Firmicute ICEs and IMEs, isolated or enclosed in composite structures.NAR genomics and bioinformatics · 2022Article
- Giant Starship Elements Mobilize Accessory Genes in Fungal Genomes.Molecular biology and evolution · 2022Article
- Prevalence and mobility of integrative and conjugative elements within aFrontiers in microbiology · 2022Article
- ComprehensiveMicrobial genomics · 2021Article
- Ecology and genomics of Actinobacteria: new concepts for natural product discovery.Nature reviews. Microbiology · 2020Review
- Replication of the Salmonella Genomic Island 1 (SGI1) triggered by helper IncC conjugative plasmids promotes incompatibility and plasmid loss.PLoS genetics · 2020Article
- Genomics of Atlantic ForestMicrobial genomics · 2020Article
- Stable Transformation of the ActinobacteriaApplied and environmental microbiology · 2019Article
- ICEberg 2.0: an updated database of bacterial integrative and conjugative elements.Nucleic acids research · 2019Article
- Regulation of Gram-Positive Conjugation.Frontiers in microbiology · 2019Review
- Mobilome ofFrontiers in microbiology · 2019Article
- Article
- Review
- De novo assembly of genomes from long sequence reads reveals uncharted territories of Propionibacterium freudenreichii.BMC genomics · 2017Article
- Integrative and conjugative elements and their hosts: composition, distribution and organization.Nucleic acids research · 2017Article
- The hidden life of integrative and conjugative elements.FEMS microbiology reviews · 2017Review
- Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Horizontal gene transfer greatly facilitates rapid genetic adaptation of bacteria to shifts in environmental conditions and colonization of new niches by allowing one-step acquisition of novel functions. Conjugation is a major mechanism of horizontal gene transfer mediated by conjugative plasmids and integrating conjugative elements (ICEs). While in most bacterial conjugative systems DNA translocation requires the assembly of a complex type IV secretion system (T4SS), in Actinobacteria a single DNA FtsK/SpoIIIE-like translocation protein is required. To date, the role and diversity of ICEs in Actinobacteria have received little attention. Putative ICEs were searched for in 275 genomes of Actinobacteria using HMM-profiles of proteins involved in ICE maintenance and transfer. These exhaustive analyses revealed 144 putative FtsK/SpoIIIE-type ICEs and 17 putative T4SS-type ICEs. Grouping of the ICEs based on the phylogenetic analyses of maintenance and transfer proteins revealed extensive exchanges between different sub-families of ICEs. 17 ICEs were found in Actinobacteria from the genus Frankia, globally important nitrogen-fixing microorganisms that establish root nodule symbioses with actinorhizal plants. Structural analysis of ICEs from Frankia revealed their unexpected diversity and a vast array of predicted adaptive functions. Frankia ICEs were found to excise by site-specific recombination from their host's chromosome in vitro and in planta suggesting that they are functional mobile elements whether Frankiae live as soil saprophytes or plant endosymbionts. Phylogenetic analyses of proteins involved in ICEs maintenance and transfer suggests that active exchange between ICEs cargo-borne and chromosomal genes took place within the Actinomycetales order. Functionality of Frankia ICEs in vitro as well as in planta lets us anticipate that conjugation and ICEs could allow the development of genetic manipulation tools for this challenging microorganism and for many other Actinobacteria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.