ArticlePLoS pathogens2011
Transcriptome analysis of Aedes aegypti transgenic mosquitoes with altered immunity.
Article in PLoS pathogens, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 140 citations in OpenAlex.
- Dual functions of the Aedes aegypti ecdysone receptor in dengue virus replication and reproduction control.Parasites & vectors · 2026Article
- Insights on the sex determination, vector capacity and ecological biology from a chromosomal level genome of vector mosquito, Armigeres subulbatus.Infectious diseases of poverty · 2025Article
- Two avian Plasmodium species trigger different transcriptional responses on their vector Culex pipiens.Molecular ecology · 2025Article
- Dynamics of vector competence for dengue virus type 2 in rural and urban populations of Aedes albopictus: implications for infectious disease control.Parasites & vectors · 2025Article
- The molecular toll pathway repertoire in anopheline mosquitoes.Developmental and comparative immunology · 2025Article
- Wolbachia infection in Aedes aegypti does not affect its vectorial capacity for Dirofilaria immitis.Scientific reports · 2024Article
- The Molecular Toll Pathway Repertoire in Anopheline Mosquitoes.bioRxiv : the preprint server for biology · 2024Article
- Innate immunity inPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- The transcriptional response in mosquitoes distinguishes between fungi and bacteria but not Gram types.BMC genomics · 2024Article
- Wolbachia infection-responsive immune genes suppress Plasmodium falciparum infection in Anopheles stephensi.PLoS pathogens · 2024Article
- Biased virus transmission following sequential coinfection of Aedes aegypti with dengue and Zika viruses.PLoS neglected tropical diseases · 2024Article
- Wolbachia-mediated resistance to Zika virus infection in Aedes aegypti is dominated by diverse transcriptional regulation and weak evolutionary pressures.PLoS neglected tropical diseases · 2023Article
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- Rift Valley Fever Virus Primes Immune Responses inPathogens (Basel, Switzerland) · 2023Article
- Transcriptome Analysis of Response to Zika Virus Infection in TwoInternational journal of molecular sciences · 2023Article
- CRISPR mediated transactivation in the human disease vector Aedes aegypti.PLoS pathogens · 2023Article
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- Aedes aegypti Malpighian tubules are immunologically activated following systemic Toll activation.Parasites & vectors · 2022Article
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- Ecdysone signaling mediates the trade-off between immunity and reproduction via suppression of amyloids in the mosquito Aedes aegypti.PLoS pathogens · 2022Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The mosquito immune system is involved in pathogen-elicited defense responses. The NF-κB factors REL1 and REL2 are downstream transcription activators of Toll and IMD immune pathways, respectively. We have used genome-wide microarray analyses to characterize fat-body-specific gene transcript repertoires activated by either REL1 or REL2 in two transgenic strains of the mosquito Aedes aegypti. Vitellogenin gene promoter was used in each transgenic strain to ectopically express either REL1 (REL1+) or REL2 (REL2+) in a sex, tissue, and stage specific manner. There was a significant change in the transcript abundance of 297 (79 up- and 218 down-regulated) and 299 (123 up- and 176 down-regulated) genes in fat bodies of REL1+ and REL2+, respectively. Over half of the induced genes had predicted functions in immunity, and a large group of these was co-regulated by REL1 and REL2. By generating a hybrid transgenic strain, which ectopically expresses both REL1 and REL2, we have shown a synergistic action of these NF-κB factors in activating immune genes. The REL1+ immune transcriptome showed a significant overlap with that of cactus (RNAi)-depleted mosquitoes (50%). In contrast, the REL2+ -regulated transcriptome differed from the relatively small group of gene transcripts regulated by RNAi depletion of a putative inhibitor of the IMD pathway, caspar (35 up- and 140 down-regulated), suggesting that caspar contributes to regulation of a subset of IMD-pathway controlled genes. Infections of the wild type Ae. aegypti with Plasmodium gallinaceum elicited the transcription of a distinct subset of immune genes (76 up- and 25 down-regulated) relative to that observed in REL1+ and REL2+ mosquitoes. Considerable overlap was observed between the fat body transcriptome of Plasmodium-infected mosquitoes and that of mosquitoes with transiently depleted PIAS, an inhibitor of the JAK-STAT pathway. PIAS gene silencing reduced Plasmodium proliferation in Ae. aegypti, indicating the involvement of the JAK-STAT pathway in anti-Plasmodium defense in this infection model.
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