ArticlePLoS pathogens2011
Indirect DNA readout by an H-NS related protein: structure of the DNA complex of the C-terminal domain of Ler.
Article in PLoS pathogens, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 61 citations in OpenAlex.
- Structural basis of the oligomerization of anti-silencer Ler from enterohemorrhagicStructural dynamics (Melville, N.Y.) · 2026Article
- Adaptation of DNA to Protein Binding Revealed by Spectroscopy and Molecular Simulation.The journal of physical chemistry. B · 2025Article
- Post-translational modifications of the nucleoid protein H-NS: sites, mechanisms, and regulatory cues.FEMS microbiology reviews · 2025Review
- Genomic island-encoded regulatory proteins in enterohemorrhagicVirulence · 2024Review
- Bacterial chromatin proteins, transcription, and DNA topology: Inseparable partners in the control of gene expression.Molecular microbiology · 2024Review
- Deciphering Sequence-Specific DNA Binding by H-NS Using Molecular Simulation.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Decoding dissociation of sequence-specific protein-DNA complexes with non-equilibrium simulations.Nucleic acids research · 2023Article
- One Earth: The Equilibrium between the Human and the Bacterial Worlds.International journal of molecular sciences · 2023Review
- ToxR activates theProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Molecular Basis for Environment Sensing by a Nucleoid-Structuring Bacterial Protein Filament.The journal of physical chemistry letters · 2021Article
- Structural basis of transcriptional activation by the Mycobacterium tuberculosis intrinsic antibiotic-resistance transcription factor WhiB7.Molecular cell · 2021Article
- PchE Regulation ofInternational journal of molecular sciences · 2020Article
- Transcription of Bacterial Chromatin.Journal of molecular biology · 2019Review
- Predicting the mechanism and rate of H-NS binding to AT-rich DNA.PLoS computational biology · 2019Article
- Identifying the region responsible for Brucella abortus MucR higher-order oligomer formation and examining its role in gene regulation.Scientific reports · 2018Article
- StpA and Hha stimulate pausing by RNA polymerase by promoting DNA-DNA bridging of H-NS filaments.Nucleic acids research · 2018Article
- Environment Controls LEE Regulation in EnteropathogenicFrontiers in microbiology · 2018Review
- Charged residues in the H-NS linker drive DNA binding and gene silencing in single cells.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Silencing of cryptic prophages in Corynebacterium glutamicum.Nucleic acids research · 2016Article
- DNA supercoiling is a fundamental regulatory principle in the control of bacterial gene expression.Biophysical reviews · 2016Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ler, a member of the H-NS protein family, is the master regulator of the LEE pathogenicity island in virulent Escherichia coli strains. Here, we determined the structure of a complex between the DNA-binding domain of Ler (CT-Ler) and a 15-mer DNA duplex. CT-Ler recognizes a preexisting structural pattern in the DNA minor groove formed by two consecutive regions which are narrower and wider, respectively, compared with standard B-DNA. The compressed region, associated with an AT-tract, is sensed by the side chain of Arg90, whose mutation abolishes the capacity of Ler to bind DNA. The expanded groove allows the approach of the loop in which Arg90 is located. This is the first report of an experimental structure of a DNA complex that includes a protein belonging to the H-NS family. The indirect readout mechanism not only explains the capacity of H-NS and other H-NS family members to modulate the expression of a large number of genes but also the origin of the specificity displayed by Ler. Our results point to a general mechanism by which horizontally acquired genes may be specifically recognized by members of the H-NS family.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.