ArticleMolecular biology of the cell2012
A new role for the architecture of microvillar actin bundles in apical retention of membrane proteins.
Article in Molecular biology of the cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
56 citing papers in PubMed, 72 citations in OpenAlex.
- Structural plasticity of mouse intestinal microvilli revealed by tissue-level cryo-ET.Biophysics reports · 2026Article
- Using CRISPR/Cas9 to investigate the role of candidate human disease gene orthologs in Ciona.bioRxiv : the preprint server for biology · 2026Article
- Synergistic assembly, disassembly, and protection of complex forms of bundled F-actin.The Journal of cell biology · 2026Article
- Fibroblast depletion reveals mammalian epithelial resilience across neonatal and adult stages.The Journal of cell biology · 2026Article
- Fibroblast depletion reveals mammalian epithelial resilience across neonatal and adult stages.bioRxiv : the preprint server for biology · 2026Article
- Pathogenic mechanism of the PLS1 gene variant in hearing loss and functional validation in a zebrafish model.Scientific reports · 2026Article
- Brush border intermicrovillar adhesion limits bacteria attachment to the small intestine brush border.bioRxiv : the preprint server for biology · 2025Article
- Apical clathrin-coated pits control the location, timing, and scale of microvillar growth.Cell reports · 2025Article
- Protrusion-Derived Extracellular Vesicles (PD-EVs) and Their Diverse Origins: Key Players in Cellular Communication, Cancer Progression, and T Cell Modulation.Biology of the cell · 2025Review
- Intestinal Tuft Cells: Morphology, Function, and Implications for Human Health.Annual review of physiology · 2024Review
- Destruction of the brush border byFrontiers in microbiology · 2024Article
- BioID2 screening identifies KIAA1671 as an EPS8 proximal factor that marks sites of microvillus growth.Molecular biology of the cell · 2023Article
- The morphological and functional diversity of apical microvilli.Journal of anatomy · 2023Review
- Actin Bundles Dynamics and Architecture.Biomolecules · 2023Review
- Building the brush border, one microvillus at a time.Current opinion in cell biology · 2023Review
- Manipulation of microvillar proteins duringFrontiers in cellular and infection microbiology · 2023Article
- The intermicrovillar adhesion complex in gut barrier function and inflammation.Exploration of digestive diseases · 2022Article
- Mitotic Spindle Positioning (MISP) is an actin bundler that selectively stabilizes the rootlets of epithelial microvilli.Cell reports · 2022Article
- Article
- Direct visualization of epithelial microvilli biogenesis.Current biology : CB · 2021Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
Abstract
Actin-bundling proteins are identified as key players in the morphogenesis of thin membrane protrusions. Until now, functional redundancy among the actin-bundling proteins villin, espin, and plastin-1 has prevented definitive conclusions regarding their role in intestinal microvilli. We report that triple knockout mice lacking these microvillar actin-bundling proteins suffer from growth delay but surprisingly still develop microvilli. However, the microvillar actin filaments are sparse and lack the characteristic organization of bundles. This correlates with a highly inefficient apical retention of enzymes and transporters that accumulate in subapical endocytic compartments. Myosin-1a, a motor involved in the anchorage of membrane proteins in microvilli, is also mislocalized. These findings illustrate, in vivo, a precise role for local actin filament architecture in the stabilization of apical cargoes into microvilli. Hence, the function of actin-bundling proteins is not to enable microvillar protrusion, as has been assumed, but to confer the appropriate actin organization for the apical retention of proteins essential for normal intestinal physiology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.