ArticleJournal of virology2012
Establishment of a novel permissive cell line for the propagation of hepatitis C virus by expression of microRNA miR122.
Article in Journal of virology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
47 citing papers in PubMed, 90 citations in OpenAlex.
- Review
- Immortalized hepatocyte-like cells: A competent hepatocyte model for studying clinical HCV isolate infection.PloS one · 2024Article
- Dichloroacetate enhances the antitumor effect of pirarubicin via regulating the ROS-JNK signaling pathway in liver cancer cells.Cancer drug resistance (Alhambra, Calif.) · 2020Article
- Establishment and Characterization of a New Cell Line Permissive for Hepatitis C Virus Infection.Scientific reports · 2019Article
- USP15 Participates in Hepatitis C Virus Propagation through Regulation of Viral RNA Translation and Lipid Droplet Formation.Journal of virology · 2019Article
- Mutational Analysis of the Bovine Hepacivirus Internal Ribosome Entry Site.Journal of virology · 2018Article
- MicroRNA Signatures for circulating CD133-positive cells in hepatocellular carcinoma with HCV infection.PloS one · 2018Article
- Engineered Livers for Infectious Diseases.Cellular and molecular gastroenterology and hepatology · 2018Review
- Establishment and evaluation of the goose embryo epithelial (GEE) cell line as a new model for propagation of avian viruses.PloS one · 2018Article
- miRNA independent hepacivirus variants suggest a strong evolutionary pressure to maintain miR-122 dependence.PLoS pathogens · 2017Article
- Characterization of miR-122-independent propagation of HCV.PLoS pathogens · 2017Article
- Quasispecies of Hepatitis C Virus Participate in Cell-Specific Infectivity.Scientific reports · 2017Article
- Human Cathelicidin Compensates for the Role of Apolipoproteins in Hepatitis C Virus Infectious Particle Formation.Journal of virology · 2016Article
- Serum microRNA panels as potential biomarkers for early detection of hepatocellular carcinoma on top of HCV infection.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- A robust model of natural hepatitis C infection using hepatocyte-like cells derived from human induced pluripotent stem cells as a long-term host.Virology journal · 2016Article
- Phosphoproteomics Identified an NS5A Phosphorylation Site Involved in Hepatitis C Virus Replication.The Journal of biological chemistry · 2016Article
- Precision multidimensional assay for high-throughput microRNA drug discovery.Nature communications · 2016Article
- Review
- Hepatitis C virus represses the cellular antiviral response by upregulating the expression of signal transducer and activator of transcription 3 through sponging microRNA‑122.Molecular medicine reports · 2015Article
- MicroRNAs: Role in Hepatitis C Virus pathogenesis.Genes & diseases · 2015Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The robust cell culture systems for hepatitis C virus (HCV) are limited to those using cell culture-adapted clones (HCV in cell culture [HCVcc]) and cells derived from the human hepatoma cell line Huh7. However, accumulating data suggest that host factors, including innate immunity and gene polymorphisms, contribute to the variation in host response to HCV infection. Therefore, the existing in vitro systems for HCV propagation are not sufficient to elucidate the life cycle of HCV. A liver-specific microRNA, miR122, has been shown to participate in the efficient replication of HCV. In this study, we examined the possibility of establishing a new permissive cell line for HCV propagation by the expression of miR122. A high level of miR122 was expressed by a lentiviral vector placed into human liver cell lines at a level comparable to the endogenous level in Huh7 cells. Among the cell lines that we examined, Hep3B cells stably expressing miR122 (Hep3B/miR122) exhibited a significant enhancement of HCVcc propagation. Surprisingly, the levels of production of infectious particles in Hep3B/miR122 cells upon infection with HCVcc were comparable to those in Huh7 cells. Furthermore, a line of "cured" cells, established by elimination of HCV RNA from the Hep3B/miR122 replicon cells, exhibited an enhanced expression of miR122 and a continuous increase of infectious titers of HCVcc in every passage. The establishment of the new permissive cell line for HCVcc will have significant implications not only for basic HCV research but also for the development of new therapeutics.
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