ArticlePlant physiology2012
Assembly of the light-harvesting chlorophyll antenna in the green alga Chlamydomonas reinhardtii requires expression of the TLA2-CpFTSY gene.
Article in Plant physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Enhancing CRISPR/Cas-Mediated Gene Knockout With Short Non-Homologous Oligonucleotides.Plant biotechnology journal · 2026Article
- Processes independent of nonphotochemical quenching protect a high-light-tolerant desert alga from oxidative stress.Plant physiology · 2024Article
- Integrative Transcriptomics and Proteomics Analysis of a Cotton MutantPlants (Basel, Switzerland) · 2024Article
- Simultaneous CAS9 editing of cpPlant direct · 2023Article
- Loss of CpFTSY Reduces Photosynthetic Performance and Affects Insertion of PsaC of PSI in Diatoms.Plant & cell physiology · 2023Article
- Improved Productivity of Astaxanthin from PhotosensitiveMarine drugs · 2022Article
- Co-targeting strategy for precise, scarless gene editing with CRISPR/Cas9 and donor ssODNs in Chlamydomonas.Plant physiology · 2021Article
- Incorporating a molecular antenna in diatom microalgae cells enhances photosynthesis.Scientific reports · 2021Article
- Cell Synchronization Enhances Nuclear Transformation and Genome EditingACS synthetic biology · 2020Article
- A point mutation in the photosystem I P700 chlorophyll a apoprotein A1 gene confers variegation in Helianthus annuus L.Plant molecular biology · 2020Article
- Consequences of the reduction of the Photosystem II antenna size on the light acclimation capacity of Arabidopsis thaliana.Plant, cell & environment · 2020Article
- Potential and Challenges of Improving Photosynthesis in Algae.Plants (Basel, Switzerland) · 2020Review
- Improved lipid productivity inBiotechnology for biofuels · 2020Article
- Site-Specific Gene Knock-Out and On-Site Heterologous Gene Overexpression inFrontiers in plant science · 2020Article
- ManiNetCluster: a novel manifold learning approach to reveal the functional links between gene networks.BMC genomics · 2019Article
- Loss of ALBINO3b Insertase Results in Truncated Light-Harvesting Antenna in Diatoms.Plant physiology · 2019Article
- Fine-tuning the photosynthetic light harvesting apparatus for improved photosynthetic efficiency and biomass yield.Scientific reports · 2019Article
- A thylakoid membrane-bound and redox-active rubredoxin (RBD1) functions in de novo assembly and repair of photosystem II.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Article
- Deletion of the chloroplast LTD protein impedes LHCI import and PSI-LHCI assembly in Chlamydomonas reinhardtii.Journal of experimental botany · 2018Article
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4 authors.
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Abstract
The truncated light-harvesting antenna2 (tla2) mutant of Chlamydomonas reinhardtii showed a lighter-green phenotype, had a lower chlorophyll (Chl) per-cell content, and higher Chl a/b ratio than corresponding wild-type strains. Physiological analyses revealed a higher intensity for the saturation of photosynthesis and greater P(max) values in the tla2 mutant than in the wild type. Biochemical analyses showed that the tla2 strain was deficient in the Chl a-b light-harvesting complex, and had a Chl antenna size of the photosystems that was only about 65% of that in the wild type. Molecular and genetic analyses showed a single plasmid insertion in the tla2 strain, causing a chromosomal DNA rearrangement and deletion/disruption of five nuclear genes. The TLA2 gene, causing the tla2 phenotype, was cloned by mapping the insertion site and upon complementation with each of the genes that were deleted. Successful complementation was achieved with the C. reinhardtii TLA2-CpFTSY gene, whose occurrence and function in green microalgae has not hitherto been investigated. Functional analysis showed that the nuclear-encoded and chloroplast-localized CrCpFTSY protein specifically operates in the assembly of the peripheral components of the Chl a-b light-harvesting antenna. In higher plants, a cpftsy null mutation inhibits assembly of both the light-harvesting complex and photosystem complexes, thus resulting in a seedling-lethal phenotype. The work shows that cpftsy deletion in green algae, but not in higher plants, can be employed to generate tla mutants. The latter exhibit improved solar energy conversion efficiency and photosynthetic productivity under mass culture and bright sunlight conditions.
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