ArticleJournal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research2012
Osteocytic network is more responsive in calcium signaling than osteoblastic network under fluid flow.
Article in Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
81 citing papers in PubMed, 137 citations in OpenAlex.
- Maintenance of bone health by osteoblasts in the absence of osteocytes: comparative analysis of gene expression in medaka and zebrafish vertebrae.Bone reports · 2026Article
- Effects of azelnidipine, a calcium channel blocker, in a rat femur fracture model.BMC musculoskeletal disorders · 2026Article
- Bone Multicellular Unit on a Chip (BMU-Chip) Subjected to Cyclic Mechanical Loading.ACS biomaterials science & engineering · 2026Article
- Immune reprogramming in the bone marrow microenvironment: a new perspective on the bone immune microenvironment of postmenopausal osteoporosis.Frontiers in immunology · 2026Review
- 3D Osteocyte Networks under Pulsatile Unidirectional Fluid Flow Stimuli (PUFFS).ACS biomaterials science & engineering · 2025Article
- Cholinergic regulation of osteocyte mechanobiology: A paradigm for bone adaptation.Science advances · 2025Article
- Ageing-Related Changes in Ultrastructural Bone Matrix Composition and Osteocyte Mechanosensitivity.Current osteoporosis reports · 2025Review
- [Relationship between fluid shear stress in alveolar bone under orthodontic forces and bone remodeling rate].Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2025Article
- Numerical Simulation of Fluid Shear Stress Distribution in Microcracks of Trabecular Bone.Applied bionics and biomechanics · 2025Article
- The roles of extracellular vesicles released by mechanically stimulated osteocytes in regulating osteoblast and osteoclast functions.Mechanobiology in medicine · 2024Article
- Fluid-solid coupling numerical simulation of the effects of different doses of verapamil on cancellous bone in type 2 diabetic rats.BMC musculoskeletal disorders · 2024Article
- Computational modeling based on confocal imaging predicts changes in osteocyte and dendrite shear stress due to canalicular loss with aging.Biomechanics and modeling in mechanobiology · 2024Article
- Loss of the auxiliary αJBMR plus · 2024Article
- Convergence of Calcium Channel Regulation and Mechanotransduction in Skeletal Regenerative Biomaterial Design.Advanced healthcare materials · 2023Review
- A Fluorescent Intravital Imaging Approach to Study Load-Induced Calcium Signaling Dynamics in Mouse Osteocytes.Journal of visualized experiments : JoVE · 2023Article
- Glucose- and glutamine-dependent bioenergetics sensitize bone mechanoresponse after unloading by modulating osteocyte calcium dynamics.The Journal of clinical investigation · 2023Article
- High Bone Mass Disorders: New Insights From Connecting the Clinic and the Bench.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2023Review
- Calcium signaling of primary chondrocytes and ATDC5 chondrogenic cells under osmotic stress and mechanical stimulation.Journal of biomechanics · 2022Article
- Impacts of aging on murine cartilage biomechanics and chondrocyte in situ calcium signaling.Journal of biomechanics · 2022Article
- The Diminishing Returns of Mechanical Loading and Potential Mechanisms that Desensitize Osteocytes.Current osteoporosis reports · 2021Review
21 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Osteocytes, regarded as the mechanical sensor in bone, respond to mechanical stimulation by activating biochemical pathways and mediating the cellular activities of other bone cells. Little is known about how osteocytic networks respond to physiological mechanical stimuli. In this study, we compared the mechanical sensitivity of osteocytic and osteoblastic networks under physiological-related fluid shear stress (0.5 to 4 Pa). The intracellular calcium ([Ca(2+)](i)) responses in micropatterned in vitro osteoblastic or osteocytic networks were recorded and analyzed. Osteocytes in the network showed highly repetitive spikelike [Ca(2+)](i) peaks under fluid flow stimulation, which are dramatically different from those in the osteoblastic network. The number of responsive osteocytes in the network remained at a constant high percentage (>95%) regardless of the magnitude of shear stress, whereas the number of responsive osteoblasts in the network significantly depends on the strength of fluid flow. All spatiotemporal parameters of calcium signaling demonstrated that osteocytic networks are more sensitive and dynamic than osteoblastic networks, especially under low-level mechanical stimulations. Furthermore, pathway studies were performed to identify the molecular mechanisms responsible for the differences in [Ca(2+)](i) signaling between osteoblastic and osteocytic networks. The results suggested that the T-type voltage-gated calcium channels (VGCC) expressed on osteocytes may play an essential role in the unique kinetics of [Ca(2+)](i) signaling in osteocytic networks, whereas the L-type VGCC is critical for both types of cells to release multiple [Ca(2+)](i) peaks. The extracellular calcium source and intracellular calcium store in ER-, ATP-, PGE₂-, NO-, and caffeine-related pathways are found to play similar roles in the [Ca(2+)](i) signaling for both osteoblasts and osteocytes. The findings in this study proved that osteocytic networks possess unique characteristics in sensing and processing mechanical signals.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.