ArticleThe ISME journal2012
Rapid resonance Raman microspectroscopy to probe carbon dioxide fixation by single cells in microbial communities.
Article in The ISME journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 110 citations in OpenAlex.
- µ-Raman and GC-MS to interrogate the heterogeneity of deuterium-label incorporation on the single cell vs. fatty acid isotopologue level.Analytical and bioanalytical chemistry · 2026Article
- Vibrational spectroscopy methodology for profiling of mixed carbon metabolism in carotenogenic and oleaginous Mucor circinelloides.Microbial cell factories · 2026Article
- Development and multi-platform validation of a pan-genomic-driven PMA-qPCR method for the precise quantification of viableFrontiers in microbiology · 2026Article
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- Raman Microspectroscopy to Trace the Incorporation of Deuterium from Labeled (Micro)Plastics into Microbial Cells.Analytical chemistry · 2025Article
- Illuminating the Tiny World: A Navigation Guide for Proper Raman Studies on Microorganisms.Molecules (Basel, Switzerland) · 2024Review
- Raman cell sorting for single-cell research.Frontiers in bioengineering and biotechnology · 2024Review
- Culture-free identification of fast-growing cyanobacteria cells by Raman-activated gravity-driven encapsulation and sequencing.Synthetic and systems biotechnology · 2023Article
- Robust Spontaneous Raman Flow Cytometry for Single-Cell Metabolic Phenome Profiling via pDEP-DLD-RFC.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Hyperglycemia and cancer in human lung carcinoma by means of Raman spectroscopy and imaging.Scientific reports · 2022Article
- Review
- Raman-Deuterium Isotope Probing and Metagenomics Reveal the Drought Tolerance of the Soil Microbiome and Its Promotion of Plant Growth.mSystems · 2022Article
- Raman Micro-spectroscopy and Imaging of Filamentous Fungi.Microbes and environments · 2022Review
- One Cell at a Time: Advances in Single-Cell Methods and Instrumentation for Discovery in Aquatic Microbiology.Frontiers in microbiology · 2022Article
- Revealing COBiodesign research · 2022Article
- Using Stable Isotope Probing and Raman Microspectroscopy To Measure Growth Rates of Heterotrophic Bacteria.Applied and environmental microbiology · 2021Article
- One-Cell Metabolic Phenotyping and Sequencing of Soil Microbiome by Raman-Activated Gravity-Driven Encapsulation (RAGE).mSystems · 2021Article
- Article
- Microbial phenomics linking the phenotype to fonction: The potential of Raman spectroscopy.Journal of microbiology (Seoul, Korea) · 2021Review
- Deuterium-labeled Raman tracking of glucose accumulation and protein metabolic dynamics in Aspergillus nidulans hyphal tips.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Photosynthetic microorganisms play crucial roles in aquatic ecosystems and are the major primary producers in global marine ecosystems. The discovery of new bacteria and microalgae that play key roles in CO(2) fixation is hampered by the lack of methods to identify hitherto-unculturable microorganisms. To overcome this problem we studied single microbial cells using stable-isotope probing (SIP) together with resonance Raman (RR) microspectroscopy of carotenoids, the light-absorbing pigments present in most photosynthetic microorganisms. We show that fixation of (13)CO(2) into carotenoids produces a red shift in single-cell RR (SCRR) spectra and that this SCRR-SIP technique is sufficiently sensitive to detect as little as 10% of (13)C incorporation. Mass spectrometry (MS) analysis of labelled cellular proteins verifies that the red shift in carotenoid SCRR spectra acts as a reporter of the (13)C content of single cells. Millisecond Raman imaging of cells in mixed cultures and natural seawater samples was used to identify cells actively fixing CO(2), demonstrating that the SCRR-SIP is a noninvasive method for the rapid and quantitative detection of CO(2) fixation at the single cell level in a microbial community. The SCRR-SIP technique may provide a direct method for screening environmental samples, and could help to reveal the ecophysiology of hitherto-unculturable microorganisms, linking microbial species to their ecological function in the natural environment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.