ArticleThe ISME journal2012
Laboratory evolution of Geobacter sulfurreducens for enhanced growth on lactate via a single-base-pair substitution in a transcriptional regulator.
Article in The ISME journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- A Multidimensional Bibliometric Analysis of Research Hotspots and Development Trends in Functional Microorganisms of the GenusMicroorganisms · 2026Review
- Environmental considerations and bioremediation applications of the metal-, nitrate-, and organohalide-respiring bacteriumApplied and environmental microbiology · 2026Review
- Propionate oxidation byApplied and environmental microbiology · 2026Article
- Influence of support materials on the electroactive behavior, structure and gene expression of wild type and GSU1771-deficient mutant of Geobacter sulfurreducens biofilms.Environmental science and pollution research international · 2025Article
- Soil microclimate and vegetation dynamics shape elevational and seasonal variations of diazotrophic communities in alpine grasslands.Frontiers in plant science · 2025Article
- Article
- Set-up of a pharmaceutical cell bank of Magnetospirillum gryphiswaldense MSR1 magnetotactic bacteria producing highly pure magnetosomes.Microbial cell factories · 2024Article
- Moving towards the enhancement of extracellular electron transfer in electrogens.World journal of microbiology & biotechnology · 2023Review
- Adapted laboratory evolution of Thermotoga sp. strain RQ7 under carbon starvation.BMC research notes · 2022Article
- Key Enzymes for Anaerobic Lactate Metabolism in Geobacter sulfurreducens.Applied and environmental microbiology · 2021Article
- Comparative proteomics of Geobacter sulfurreducens PCAEnvironmental microbiology · 2021Article
- The hidden chemolithoautotrophic metabolism of Geobacter sulfurreducens uncovered by adaptation to formate.The ISME journal · 2020Article
- Article
- The emergence of adaptive laboratory evolution as an efficient tool for biological discovery and industrial biotechnology.Metabolic engineering · 2019Review
- Long-Term Behavior of Defined Mixed Cultures ofFrontiers in bioengineering and biotechnology · 2019Article
- Article
- Resilience, Dynamics, and Interactions within a Model Multispecies Exoelectrogenic-Biofilm Community.Applied and environmental microbiology · 2017Article
- Rapid selective sweep of pre-existing polymorphisms and slow fixation of new mutations in experimental evolution of Desulfovibrio vulgaris.The ISME journal · 2015Article
- Scarless Genome Editing and Stable Inducible Expression Vectors for Geobacter sulfurreducens.Applied and environmental microbiology · 2015Article
- Adaptive laboratory evolution -- principles and applications for biotechnology.Microbial cell factories · 2013Review
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The addition of organic compounds to groundwater in order to promote bioremediation may represent a new selective pressure on subsurface microorganisms. The ability of Geobacter sulfurreducens, which serves as a model for the Geobacter species that are important in various types of anaerobic groundwater bioremediation, to adapt for rapid metabolism of lactate, a common bioremediation amendment, was evaluated. Serial transfer of five parallel cultures in a medium with lactate as the sole electron donor yielded five strains that could metabolize lactate faster than the wild-type strain. Genome sequencing revealed that all five strains had non-synonymous single-nucleotide polymorphisms in the same gene, GSU0514, a putative transcriptional regulator. Introducing the single-base-pair mutation from one of the five strains into the wild-type strain conferred rapid growth on lactate. This strain and the five adaptively evolved strains had four to eight-fold higher transcript abundance than wild-type cells for genes for the two subunits of succinyl-CoA synthase, an enzyme required for growth on lactate. DNA-binding assays demonstrated that the protein encoded by GSU0514 bound to the putative promoter of the succinyl-CoA synthase operon. The binding sequence was not apparent elsewhere in the genome. These results demonstrate that a single-base-pair mutation in a transcriptional regulator can have a significant impact on the capacity for substrate utilization and suggest that adaptive evolution should be considered as a potential response of microorganisms to environmental change(s) imposed during bioremediation.
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