ArticleGenome biology2011
Heat shock factor binding in Alu repeats expands its involvement in stress through an antisense mechanism.
Article in Genome biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 44 citations in OpenAlex.
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- Mobility connects: transposable elements wire new transcriptional networks by transferring transcription factor binding motifs.Biochemical Society transactions · 2020Review
- Not All Stressors Are Equal: Mechanism of Stressors on RPE Cell Degeneration.Frontiers in cell and developmental biology · 2020Review
- The CLN3 gene and protein: What we know.Molecular genetics & genomic medicine · 2019Review
- Alu RNA accumulation induces epithelial-to-mesenchymal transition by modulating miR-566 and is associated with cancer progression.Oncogene · 2018Article
- Putative RNA-directed adaptive mutations in cancer evolution.Transcription · 2016Article
- Alu-miRNA interactions modulate transcript isoform diversity in stress response and reveal signatures of positive selection.Scientific reports · 2016Article
- lncRNAs in Stress Response.Current topics in microbiology and immunology · 2016Review
- Short interspersed DNA elements and miRNAs: a novel hidden gene regulation layer in zebrafish?Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Article
- CGGBP1--an indispensable protein with ubiquitous cytoprotective functions.Upsala journal of medical sciences · 2015Review
- Prion-like nuclear aggregation of TDP-43 during heat shock is regulated by HSP40/70 chaperones.Human molecular genetics · 2014Article
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- Transcriptome-wide expansion of non-coding regulatory switches: evidence from co-occurrence of Alu exonization, antisense and editing.Nucleic acids research · 2013Article
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- Cellular response to heat shock studied by multiconfocal fluorescence correlation spectroscopy.Biophysical journal · 2012Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlu RNAs are present at elevated levels in stress conditions and, consequently, Alu repeats are increasingly being associated with the physiological stress response. Alu repeats are known to harbor transcription factor binding sites that modulate RNA pol II transcription and Alu RNAs act as transcriptional co-repressors through pol II binding in the promoter regions of heat shock responsive genes. An observation of a putative heat shock factor (HSF) binding site in Alu led us to explore whether, through HSF binding, these elements could further contribute to the heat shock response repertoire.
resultsAlu density was significantly enriched in transcripts that are down-regulated following heat shock recovery in HeLa cells. ChIP analysis confirmed HSF binding to a consensus motif exhibiting positional conservation across various Alu subfamilies, and reporter constructs demonstrated a sequence-specific two-fold induction of these sites in response to heat shock. These motifs were over-represented in the genic regions of down-regulated transcripts in antisense oriented Alus. Affymetrix Exon arrays detected antisense signals in a significant fraction of the down-regulated transcripts, 50% of which harbored HSF sites within 5 kb. siRNA knockdown of the selected antisense transcripts led to the over-expression, following heat shock, of their corresponding down-regulated transcripts. The antisense transcripts were significantly enriched in processes related to RNA pol III transcription and the TFIIIC complex.
conclusionsWe demonstrate a non-random presence of Alu repeats harboring HSF sites in heat shock responsive transcripts. This presence underlies an antisense-mediated mechanism that represents a novel component of Alu and HSF involvement in the heat shock response.
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