ArticlePloS one2011
Photoporation of biomolecules into single cells in living vertebrate embryos induced by a femtosecond laser amplifier.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Spatially-Resolved Organoid Transfection by Porous Silicon-Mediated Optoporation.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- Lipids and Lipid Derivatives for RNA Delivery.Chemical reviews · 2021Review
- Intracellular Delivery of mRNA in Adherent and Suspension Cells by Vapor Nanobubble Photoporation.Nano-micro letters · 2020Article
- AAV diffuses across zona pellucida for effortless gene delivery to fertilized eggs.Biochemical and biophysical research communications · 2020Article
- Enzyme-Assisted Photoinjection of Megadalton Molecules into Intact Plant Cells Using Femtosecond Laser Amplifier.Scientific reports · 2019Article
- Laser-assisted Lentiviral Gene Delivery to Mouse Fertilized Eggs.Journal of visualized experiments : JoVE · 2018Article
- Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.Chemical reviews · 2018Review
- Targeted delivery of fluorogenic peptide aptamers into live microalgae by femtosecond laser photoporation at single-cell resolution.Scientific reports · 2018Article
- En masse lentiviral gene delivery to mouse fertilized eggs via laser perforation of zona pellucida.Transgenic research · 2018Article
- 18 GHz electromagnetic field induces permeability of Gram-positive cocci.Scientific reports · 2015Article
- Live imaging of calcium spikes during double fertilization in Arabidopsis.Nature communications · 2014Article
- Femtosecond optical transfection of individual mammalian cells.Nature protocols · 2013Article
- Cell signaling experiments driven by optical manipulation.International journal of molecular sciences · 2013Review
- Statistical organelle dissection of Arabidopsis guard cells using image database LIPS.Scientific reports · 2012Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Introduction of biomolecules into cells in living animals is one of the most important techniques in molecular and developmental biology research, and has potentially broad biomedical implications. Here we report that biomolecules can be introduced into single cells in living vertebrate embryos by photoporation using a femtosecond laser amplifier with a high pulse energy and a low repetition rate. First, we confirmed the efficiency of this photoporation technique by introducing dextran, morpholino oligonucleotides, or DNA plasmids into targeted single cells of zebrafish, chick, shark, and mouse embryos. Second, we demonstrated that femtosecond laser irradiation efficiently delivered DNA plasmids into single neurons of chick embryos. Finally, we successfully manipulated the fate of single neurons in zebrafish embryos by delivering mRNA. Our observations suggest that photoporation using a femtosecond laser with a high pulse energy and low repetition rate offers a novel way to manipulate the function(s) of individual cells in a wide range of vertebrate embryos by introduction of selected biomolecules.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.