ArticlePloS one2011
A conserved GA element in TATA-less RNA polymerase II promoters.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- Core promoterome of barley embryo.Computational and structural biotechnology journal · 2024Article
- Broad compatibility between yeast UAS elements and core promoters and identification of promoter elements that determine cofactor specificity.Cell reports · 2023Article
- Large-scale analysis of Drosophila core promoter function using synthetic promoters.Molecular systems biology · 2022Article
- Genome-Wide Regulations of the Preinitiation Complex Formation and Elongating RNA Polymerase II by an E3 Ubiquitin Ligase, San1.Molecular and cellular biology · 2022Article
- The origin and evolution of a distinct mechanism of transcription initiation in yeasts.Genome research · 2021Article
- Universal promoter scanning by Pol II during transcription initiation in Saccharomyces cerevisiae.Genome biology · 2020Article
- A 14-bp stretch plays a critical role in regulating gene expression from βCurrent genetics · 2020Article
- Pervasive and dynamic transcription initiation inGenome research · 2019Article
- Recent advances in the applications of promoter engineering for the optimization of metabolite biosynthesis.World journal of microbiology & biotechnology · 2019Review
- Mechanistic Differences in Transcription Initiation at TATA-Less and TATA-Containing Promoters.Molecular and cellular biology · 2018Article
- Synthetic Core Promoters as Universal Parts for Fine-Tuning Expression in Different Yeast Species.ACS synthetic biology · 2017Article
- Article
- Core promoter sequence in yeast is a major determinant of expression level.Genome research · 2015Article
- Article
- Mutations on the DNA binding surface of TBP discriminate between yeast TATA and TATA-less gene transcription.Molecular and cellular biology · 2014Article
- The conformational state of the nucleosome entry-exit site modulates TATA box-specific TBP binding.Nucleic acids research · 2014Article
- Mediator phosphorylation prevents stress response transcription during non-stress conditions.The Journal of biological chemistry · 2012Article
- Structure of the Mediator head module.Nature · 2012Article
- Purification and characterization of transcription factors.Mass spectrometry reviewsReview
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Initiation of RNA polymerase (Pol) II transcription requires assembly of the pre-initiation complex (PIC) at the promoter. In the classical view, PIC assembly starts with binding of the TATA box-binding protein (TBP) to the TATA box. However, a TATA box occurs in only 15% of promoters in the yeast Saccharomyces cerevisiae, posing the question how most yeast promoters nucleate PIC assembly. Here we show that one third of all yeast promoters contain a novel conserved DNA element, the GA element (GAE), that generally does not co-occur with the TATA box. The distance of the GAE to the transcription start site (TSS) resembles the distance of the TATA box to the TSS. The TATA-less TMT1 core promoter contains a GAE, recruits TBP, and supports formation of a TBP-TFIIB-DNA-complex. Mutation of the promoter region surrounding the GAE abolishes transcription in vivo and in vitro. A 32-nucleotide promoter region containing the GAE can functionally substitute for the TATA box in a TATA-containing promoter. This identifies the GAE as a conserved promoter element in TATA-less promoters.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.