ArticlePloS one2011
Long-term sphere culture cannot maintain a high ratio of cancer stem cells: a mathematical model and experiment.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- Cancer stem cell theory: Are we moving past the mist?Journal of the Chinese Medical Association : JCMA · 2019Review
- The stem cell markers Oct4A, Nanog and c-Myc are expressed in ascites cells and tumor tissue of ovarian cancer patients.Cellular oncology (Dordrecht, Netherlands) · 2013Article
- Three-dimensional-engineered matrix to study cancer stem cells and tumorsphere formation: effect of matrix modulus.Tissue engineering. Part A · 2013Article
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5 authors.
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Abstract
Acquiring abundant and high-purity cancer stem cells (CSCs) is an important prerequisite for CSC research. At present, researchers usually gain high-purity CSCs through flow cytometry sorting and expand them by short-term sphere culture. However, it is still uncertain whether we can amplify high-purity CSCs through long-term sphere culture. We have proposed a mathematical model using ordinary differential equations to derive the continuous variation of the CSC ratio in long-term sphere culture and estimated the model parameters based on a long-term sphere culture of MCF-7 stem cells. We found that the CSC ratio in long-term sphere culture presented as gradually decreased drift and might be stable at a lower level. Furthermore, we found that fitted model parameters could explain the main growth pattern of CSCs and differentiated cancer cells in long-term sphere culture.
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