ArticleThe Journal of biological chemistry2012
Specific armadillo repeat sequences facilitate β-catenin nuclear transport in live cells via direct binding to nucleoporins Nup62, Nup153, and RanBP2/Nup358.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 37 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 76 citations in OpenAlex.
- Exploring Cellular Gateways: Unraveling the Secrets of Disordered Proteins within Live Nuclear Pores.Research square · 2024Article
- Oxoglutarate dehydrogenase-like inhibits the progression of hepatocellular carcinoma by inducing DNA damage through non-canonical function.Cell death and differentiation · 2023Article
- Wnt Signaling Inhibitors and Their Promising Role in Tumor Treatment.International journal of molecular sciences · 2023Review
- Types of nuclear localization signals and mechanisms of protein import into the nucleus.Cell communication and signaling : CCS · 2021Review
- Direct interaction of β-catenin with nuclear ESM1 supports stemness of metastatic prostate cancer.The EMBO journal · 2021Article
- Emerging Gene-Editing Modalities for Osteoarthritis.International journal of molecular sciences · 2020Review
- DNA methylation in Hepatoblastoma-a literature review.Italian journal of pediatrics · 2020Review
- Retinal Physiology and Circulation: Effect of Diabetes.Comprehensive Physiology · 2020Review
- Hyperglycemia-induced ubiquitination and degradation of β-catenin with the loss of platelet endothelial cell adhesion molecule-1 in retinal endothelial cells.Microcirculation (New York, N.Y. : 1994) · 2020Article
- Gene regulation through dynamic actin control of nuclear structure.Experimental biology and medicine (Maywood, N.J.) · 2019Review
- Wnt/β-Catenin Signaling in Liver Cancers.Cancers · 2019Review
- Sun-mediated mechanical LINC between nucleus and cytoskeleton regulates βcatenin nuclear access.Journal of biomechanics · 2018Article
- Review
- Action and function of Wnt/β-catenin signaling in the progression from chronic hepatitis C to hepatocellular carcinoma.Journal of gastroenterology · 2017Review
- Self-regulation of functional pathways by motifs inside the disordered tails of beta-catenin.BMC genomics · 2016Article
- Concise Review: Plasma and Nuclear Membranes Convey Mechanical Information to Regulate Mesenchymal Stem Cell Lineage.Stem cells (Dayton, Ohio) · 2016Review
- 5-Hydroxytryptamine promotes hepatocellular carcinoma proliferation by influencing β-catenin.Molecular oncology · 2016Article
- NAG-1/GDF15 accumulates in the nucleus and modulates transcriptional regulation of the Smad pathway.Oncogene · 2016Article
- Ubiquitin specific protease 4 positively regulates the WNT/β-catenin signaling in colorectal cancer.Molecular oncology · 2015Article
- Wnt ligand-dependent activation of the negative feedback regulator Nkd1.Molecular biology of the cell · 2015Article
Corrections and comments
- Retraction · 2016-02-26Error in Data · Error in Image · Manipulation of Images ·
- Retracted
Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
β-Catenin transduces the Wnt signal from the membrane to nucleus, and certain gene mutations trigger its nuclear accumulation leading to cell transformation and cancer. β-Catenin shuttles between the nucleus and cytoplasm independent of classical Ran/transport receptor pathways, and this movement was previously hypothesized to involve the central Armadillo (Arm) domain. Fluorescence recovery after photobleaching (FRAP) assays were used to delineate functional transport regions of the Arm domain in living cells. The strongest nuclear import/export activity was mapped to Arm repeats R10-12 using both in vivo FRAP and in vitro export assays. By comparison, Arm repeats R3-8 of β-catenin were highly active for nuclear import but displayed a comparatively weak export activity. We show for the first time using purified components that specific Arm sequences of β-catenin interact directly in vitro with the FG repeats of the nuclear pore complex (NPC) components Nup62, Nup98, and Nup153, indicating an independent ability of β-catenin to traverse the NPC. Moreover, a proteomics screen identified RanBP2/Nup358 as a binding partner of Arm R10-12, and β-catenin was confirmed to interact with endogenous and ectopic forms of Nup358. We further demonstrate that knock-down of endogenous Nup358 and Nup62 impeded the rate of nuclear import/export of β-catenin to a greater extent than that of importin-β. The Arm R10-12 sequence facilitated transport even when β-catenin was bound to the Arm-binding partner LEF-1, and its activity was stimulated by phosphorylation at Tyr-654. These findings provide functional evidence that the Arm domain contributes to regulated β-catenin transport through direct interaction with the NPC.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.