ArticleInvestigative ophthalmology & visual science2011
Role of β-adrenergic receptor regulation of TNF-α and insulin signaling in retinal Muller cells.
Article in Investigative ophthalmology & visual science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 34 citations in OpenAlex.
- BetaScandinavian journal of medicine & science in sports · 2022Trial
- Cell Sources for Retinal Regeneration: Implication for Data Translation in Biomedicine of the Eye.Cells · 2022Review
- Mechanisms of β-adrenergic receptors agonists in mediating pro and anti-apoptotic pathways in hyperglycemic Müller cells.Molecular biology reports · 2022Article
- Neurobiology of resilience in depression: immune and vascular insights from human and animal studies.The European journal of neuroscience · 2021Review
- A Milled Microdevice to Advance Glia-Mediated Therapies in the Adult Nervous System.Micromachines · 2019Article
- HMGB1 inhibits insulin signalling through TLR4 and RAGE in human retinal endothelial cells.Growth factors (Chur, Switzerland) · 2018Article
- miRNA15a regulates insulin signal transduction in the retinal vasculature.Cellular signalling · 2018Article
- Toll-like receptor 4 regulates insulin signal transduction in retinal Müller cells.Growth factors (Chur, Switzerland) · 2017Article
- TLR4 regulates insulin-resistant proteins to increase apoptosis in the mouse retina.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2017Article
- Müller cells and diabetic retinopathy.Vision research · 2017Review
- Loss of TLR4 in mouse Müller cells inhibits both MyD88-dependent and -independent signaling.PloS one · 2017Article
- A regulatory role for β-adrenergic receptors regarding the resolvin D1 (RvD1) pathway in the diabetic retina.PloS one · 2017Article
- IRS proteins and diabetic complications.Diabetologia · 2016Review
- β-Adrenergic receptor agonist, compound 49b, inhibits TLR4 signaling pathway in diabetic retina.Immunology and cell biology · 2016Article
- High constitutive Akt2 activity in U937 promonocytes: effective reduction of Akt2 phosphorylation by the histamine H2-receptor and the β2-adrenergic receptor.Naunyn-Schmiedeberg's archives of pharmacology · 2016Article
- Sodium salicylate reduced insulin resistance in the retina of a type 2 diabetic rat model.PloS one · 2015Article
- Pioglitazone normalizes insulin signaling in the diabetic rat retina through reduction in tumor necrosis factor α and suppressor of cytokine signaling 3.The Journal of biological chemistry · 2014Article
- Etanercept restores normal insulin signal transduction in β2-adrenergic receptor knockout mice.Journal of neuroinflammation · 2014Article
- Intravitreal injection of IGFBP-3 restores normal insulin signaling in diabetic rat retina.PloS one · 2014Article
- Serine 307 on insulin receptor substrate 1 is required for SOCS3 and TNF-α signaling in the rMC-1 cell line.Molecular vision · 2014Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
purposeThe goal of this study was to determine the relationship of TNF-α and the downregulation of insulin receptor signaling in retinal Müller cells cultured under hyperglycemic conditions and the role of β-adrenergic receptors in regulating these responses.
methodsRetinal Müller cells were cultured in normal (5 mM) or high (25 mM) glucose until 80% confluent and then were reduced to 2% serum for 18 to 24 hours. The cells were then treated with 10 μM salmeterol followed by Western blot analysis or ELISA. For TNF-α inhibitory studies, the cells were treated with 5 ng/mL of TNF-α for 30 minutes or by a 30-minute pretreatment with TNF-α followed by salmeterol for 6 hours. In the TNF-α short hairpin (sh)RNA experiments, the cells were cultured until 90% confluent, followed by transfection with TNF-α shRNA for 18 hours.
resultsTNF-α-only treatments of Müller cells resulted in significant decreases of tyrosine phosphorylation of the insulin receptor and Akt in high-glucose conditions. Salmeterol (10 μM), a β-2-adrenergic receptor agonist, significantly increased phosphorylation of both insulin receptor and Akt. TNF-α shRNA significantly decreased phosphorylation of IRS-1(Ser307), which was further decreased after salmeterol+TNF-α shRNA. Both TNF-α shRNA and salmeterol significantly reduced death of the retinal Müller cells.
conclusionsThese studies demonstrate that β-adrenergic receptor agonists in vitro can restore the loss of insulin receptor activity noted in diabetes. By decreasing the levels of TNF-α and decreasing the phosphorylation of IRS-1(Ser307) while increasing tyrosine phosphorylation of insulin receptor, these results suggest a possible mechanism by which restoration of β-adrenergic receptor signaling may protect the retina against diabetes-induced damage.
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