ArticlePlant cell reports2012
Interspecific somatic hybrids between Cyclamen persicum and C. coum, two sexually incompatible species.
Article in Plant cell reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- An Introduction to Plant Cell, Tissue, and Organ Culture: Current Status and Perspectives.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Tissue Culture in Ornamentals: Cultivation Factors, Propagation Techniques, and Its Application.Plants (Basel, Switzerland) · 2022Review
- Real-time detection of somatic hybrid cells during electrofusion of carrot protoplasts with stably labelled mitochondria.Scientific reports · 2020Article
- Towards development of new ornamental plants: status and progress in wide hybridization.Planta · 2016Review
- Water consumption and biomass production of protoplast fusion lines of poplar hybrids under drought stress.Frontiers in plant science · 2015Article
- Toward breeding new land-sea plant hybrid species irrigable with seawater for dry regions.Plant signaling & behavior · 2015Article
- Progress in plant protoplast research.Planta · 2013Review
- Identification and characterization of a novel bipartite nuclear localization signal in the hepatitis B virus polymerase.World journal of gastroenterology · 2013Article
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Authors and funding
5 authors.
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Abstract
By applying polyethylene glycol (PEG)-mediated protoplast fusion, the first somatic hybrids were obtained between Cyclamen persicum (2n = 2x = 48) and C. coum (2n = 2x = 30)-two species that cannot be combined by cross breeding. Heterofusion was detected by double fluorescent staining with fluorescein diacetate and scopoletin. The highest heterofusion frequencies (of about 5%) resulted from a protocol using a protoplast density of 1 × 10(6)/mL and 40% PEG. The DNA content of C. coum was estimated for the first time by propidium iodide staining to be 14.7 pg/2C and was 4.6 times higher than that of C. persicum. Among 200 in vitro plantlets regenerated from fusion experiments, most resembled the C. coum parent, whereas only 5 plants showed typical C. persicum phenotypes and 46 had a deviating morphology. By flow cytometry, six putative somatic hybrids were identified. A species-specific DNA marker was developed based on the sequence of the 5.8S gene in the ribosomal nuclear DNA and its flanking internal transcribed spacers ITS1 and ITS2. The hybrid status of only one plant could be verified by the species-specific DNA marker as well as sequencing of the amplification product. RAPD markers turned out to be less informative and applicable for hybrid identification, as no clear additivity of the parental marker bands was observed. Chromosome counting in root tips of four hybrids revealed the presence of the 30 C. coum chromosomes and 2-41 additional ones indicating elimination of C. persicum chromosomes.
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