ArticleMolecular & cellular proteomics : MCP2012
Dynamics of the G protein-coupled vasopressin V2 receptor signaling network revealed by quantitative phosphoproteomics.
Article in Molecular & cellular proteomics : MCP, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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46 citing papers in PubMed, 78 citations in OpenAlex.
- Bayesian mapping of protein kinases to vasopressin-regulated phosphorylation sites in renal collecting duct.American journal of physiology. Renal physiology · 2024Article
- Vascular Dysfunction in Preeclampsia.Cells · 2021Review
- Phosphoproteomic Identification of Vasopressin/cAMP/Protein Kinase A-Dependent Signaling in Kidney.Molecular pharmacology · 2021Review
- Phosphoproteomic identification of vasopressin-regulated protein kinases in collecting duct cells.British journal of pharmacology · 2021Article
- The Biology of Vasopressin.Biomedicines · 2021Review
- Urinary exosome proteomic profiling defines stage-specific rapid progression of autosomal dominant polycystic kidney disease and tolvaptan efficacy.BBA advances · 2021Article
- Regulation of cardiac O-GlcNAcylation: More than just nutrient availability.Biochimica et biophysica acta. Molecular basis of disease · 2020Review
- New insights into the transcriptional regulation of aquaporin-2 and the treatment of X-linked hereditary nephrogenic diabetes insipidus.Kidney research and clinical practice · 2019Article
- Germline-Derived Gain-of-Function Variants of GsJournal of the American Society of Nephrology : JASN · 2019Article
- From Molecules to Mechanisms: Functional Proteomics and Its Application to Renal Tubule Physiology.Physiological reviews · 2018Review
- Sequence-based searching of custom proteome and transcriptome databases.Physiological reports · 2018Article
- In vivo brain GPCR signaling elucidated by phosphoproteomics.Science (New York, N.Y.) · 2018Article
- Identification of UT-A1- and AQP2-interacting proteins in rat inner medullary collecting duct.American journal of physiology. Cell physiology · 2018Article
- Identification of β-catenin-interacting proteins in nuclear fractions of native rat collecting duct cells.American journal of physiology. Renal physiology · 2017Article
- From 20th century metabolic wall charts to 21st century systems biology: database of mammalian metabolic enzymes.American journal of physiology. Renal physiology · 2017Article
- Activation of the metabolic sensor AMP-activated protein kinase inhibits aquaporin-2 function in kidney principal cells.American journal of physiology. Renal physiology · 2016Article
- Signaling through hepatocyte vasopressin receptor 1 protects mouse liver from ischemia-reperfusion injury.Oncotarget · 2016Article
- Comprehensive database of human E3 ubiquitin ligases: application to aquaporin-2 regulation.Physiological genomics · 2016Article
- Proteomic profiling of nuclear fractions from native renal inner medullary collecting duct cells.Physiological genomics · 2016Article
- Characterization of AQPs in Mouse, Rat, and Human Colon and Their Selective Regulation by Bile Acids.Frontiers in nutrition · 2016Article
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6 authors at 2 institutions in 1 country.
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Abstract
G protein-coupled receptors (GPCRs) regulate diverse physiological processes, and many human diseases are due to defects in GPCR signaling. To identify the dynamic response of a signaling network downstream from a prototypical G(s)-coupled GPCR, the vasopressin V2 receptor, we have carried out multireplicate, quantitative phosphoproteomics with iTRAQ labeling at four time points following vasopressin exposure at a physiological concentration in cells isolated from rat kidney. A total of 12,167 phosphopeptides were identified from 2,783 proteins, with 273 changing significantly in abundance with vasopressin. Two-dimensional clustering of phosphopeptide time courses and Gene Ontology terms revealed that ligand binding to the V2 receptor affects more than simply the canonical cyclic adenosine monophosphate-protein kinase A and arrestin pathways under physiological conditions. The regulated proteins included key components of actin cytoskeleton remodeling, cell-cell adhesion, mitogen-activated protein kinase signaling, Wnt/β-catenin signaling, and apoptosis pathways. These data suggest that vasopressin can regulate an array of cellular functions well beyond its classical role in regulating water and solute transport. These results greatly expand the current view of GPCR signaling in a physiological context and shed new light on potential roles for this signaling network in disorders such as polycystic kidney disease. Finally, we provide an online resource of physiologically regulated phosphorylation sites with dynamic quantitative data (http://helixweb.nih.gov/ESBL/Database/TiPD/index.html).
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