ArticleBMC plant biology2011
EST sequencing and gene expression profiling of defence-related genes from Persea americana infected with Phytophthora cinnamomi.
Article in BMC plant biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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20 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
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- Dual RNA-Sequencing Analysis of Resistant (International journal of molecular sciences · 2021Article
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- Mild water stress-induced priming enhance tolerance to Rosellinia necatrix in susceptible avocado rootstocks.BMC plant biology · 2019Article
- Primary Metabolism in Avocado Fruit.Frontiers in plant science · 2019Review
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- The effect on the transcriptome of Anemone coronaria following infection with rust (Tranzschelia discolor).PloS one · 2015Article
- Phylogenetic and expression analysis of the NPR1-like gene family from Persea americana (Mill.).Frontiers in plant science · 2015Article
- Sequence diversity in the large subunit of RNA polymerase I contributes to Mefenoxam insensitivity in Phytophthora infestans.Molecular plant pathology · 2014Article
- Flowering as the most highly sensitive period of grapevine (Vitis vinifera L. cv Mourvèdre) to the Botryosphaeria dieback agents Neofusicoccum parvum and Diplodia seriata infection.International journal of molecular sciences · 2014Article
- Transcriptome analysis of differentially expressed genes relevant to variegation in peach flowers.PloS one · 2014Article
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- EST sequencing and gene expression profiling in Scutellaria baicalensis.EXCLI journal · 2014Article
- Transcriptional profiling of Zea mays roots reveals roles for jasmonic acid and terpenoids in resistance against Phytophthora cinnamomi.Functional & integrative genomics · 2013Article
- Analysis of the leaf transcriptome of Musa acuminata during interaction with Mycosphaerella musicola: gene assembly, annotation and marker development.BMC genomics · 2013Article
- Transcriptome analysis of the differentially expressed genes in the male and female shrub willows (Salix suchowensis).PloS one · 2013Article
- Small RNA and transcriptome deep sequencing proffers insight into floral gene regulation in Rosa cultivars.BMC genomics · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAvocado (Persea americana) belongs to the Lauraceae family and is an important commercial fruit crop in over 50 countries. The most serious pathogen affecting avocado production is Phytophthora cinnamomi which causes Phytophthora root rot (PRR). Root pathogens such as P. cinnamomi and their interactions with hosts are poorly understood and despite the importance of both the avocado crop and the effect Phytophthora has on its cultivation, there is a lack of molecular knowledge underpinning our understanding of defence strategies against the pathogen. In order to initiate a better understanding of host-specific defence we have generated EST data using 454 pyrosequencing and profiled nine defence-related genes from Pc-infected avocado roots.
results2.0 Mb of data was generated consisting of ~10,000 reads on a single lane of the GS FLX platform. Using the Newbler assembler 371 contigs were assembled, of which 367 are novel for Persea americana. Genes were classified according to Gene Ontology terms. In addition to identifying root-specific ESTs we were also able to identify and quantify the expression of nine defence-related genes that were differentially regulated in response to P. cinnamomi. Genes such as metallothionein, thaumatin and the pathogenesis related PsemI, mlo and profilin were found to be differentially regulated.
conclusionsThis is the first study in elucidating the avocado root transcriptome as well as identifying defence responses of avocado roots to the root pathogen P. cinnamomi. Our data is currently the only EST data that has been generated for avocado rootstocks, and the ESTs identified in this study have already been useful in identifying defence-related genes as well as providing gene information for other studies looking at processes such as ROS regulation as well as hypoxia in avocado roots. Our EST data will aid in the elucidation of the avocado transcriptome and identification of markers for improved rootstock breeding and screening. The characterization of the avocado transcriptome will furthermore form a basis for functional genomics of basal angiosperms.
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