ArticleProceedings of the National Academy of Sciences of the United States of America2011
DNA double-strand breaks induced by high NaCl occur predominantly in gene deserts.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it, 54 citations in OpenAlex.
- XRCC1 Arg399Gln polymorphism is not associated with oral cancer risk: evidence from a meta-analysis.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2014Pooled it
- The meiotic achilles' heel: vulnerability and resilience under environmental stress.Protoplasma · 2026Review
- Hologenomic rewiring facilitates dietary adaptation to chitin-rich marine resources in the crab-eating frog.Frontiers in zoology · 2026Article
- From high-salt diet to cancer immunity: gut microbiota and immune system interactions and their mechanisms.Frontiers in immunology · 2026Review
- Effects of various physicochemical parameters on the Biofilm formation and Pyocyanin production in Pseudomonas aeruginosa PA14.Biotechnology letters · 2025Article
- Nutritional supplement containing a nuclear fraction of bovine thymus gland increases the circulating levels of spermidine.PloS one · 2025Article
- Exploring the complexity of genome size reduction in angiosperms.Plant molecular biology · 2024Review
- DNA break induces rapid transcription repression mediated by proteasome-dependent RNAPII removal.Cell reports · 2024Article
- Long-term health outcomes associated with hydration status.Nature reviews. Nephrology · 2024Review
- Assessing the feasibility of free DNA for disaster victim identification and forensic applications.Scientific reports · 2024Article
- The Link between Autosomal Dominant Polycystic Kidney Disease and Chromosomal Instability: Exploring the Relationship.International journal of molecular sciences · 2024Article
- Single-cell analysis in hypersaline brines predicts a water-activity limit of microbial anabolic activity.Science advances · 2023Article
- Behavioural changes in slime moulds over time.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2023Article
- Middle-age high normal serum sodium as a risk factor for accelerated biological aging, chronic diseases, and premature mortality.EBioMedicine · 2023Article
- Single-Cell Analysis of CHO Cells Reveals Clonal Heterogeneity in Hyperosmolality-Induced Stress Response.Cells · 2022Article
- Tissue Sodium Accumulation: Pathophysiology and Clinical Implications.Antioxidants (Basel, Switzerland) · 2022Review
- Hyperosmolality in CHO cell culture: effects on the proteome.Applied microbiology and biotechnology · 2022Article
- High salt intake combined with hypertension elevated the risk of primary liver cancer: a prospective cohort study.Frontiers in oncology · 2022Article
- Detection and quantification of γ-H2AX using a dissociation enhanced lanthanide fluorescence immunoassay.Scientific reports · 2021Article
- Cellular ATP Levels Determine the Stability of a Nucleotide Kinase.Frontiers in molecular biosciences · 2021Article
Corrections and comments
- Commented on by
Authors and funding
5 authors at 4 institutions in 1 country.
Funding
Abstract
High concentration of NaCl increases DNA breaks both in cell culture and in vivo. The breaks remain elevated as long as NaCl concentration remains high and are rapidly repaired when the concentration is lowered. The exact nature of the breaks, and their location, has not been entirely clear, and it has not been evident how cells survive, replicate, and maintain genome integrity in environments like the renal inner medulla in which cells are constantly exposed to high NaCl concentration. Repair of the breaks after NaCl is reduced is accompanied by formation of foci containing phosphorylated H2AX (γH2AX), which occurs around DNA double-strand breaks and contributes to their repair. Here, we confirm by specific comet assay and pulsed-field electrophoresis that cells adapted to high NaCl have increased levels of double-strand breaks. Importantly, γH2AX foci that occur during repair of the breaks are nonrandomly distributed in the mouse genome. By chromatin immunoprecipitation using anti-γH2AX antibody, followed by massive parallel sequencing (ChIP-Seq), we find that during repair of double-strand breaks induced by high NaCl, γH2AX is predominantly localized to regions of the genome devoid of genes ("gene deserts"), indicating that the high NaCl-induced double-strand breaks are located there. Localization to gene deserts helps explain why the DNA breaks are less harmful than are the random breaks induced by genotoxic agents such as UV radiation, ionizing radiation, and oxidants. We propose that the universal presence of NaCl around animal cells has directly influenced the evolution of the structure of their genomes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.