ArticleProceedings of the National Academy of Sciences of the United States of America2011
Single-molecule protein arrays enabled by scanning probe block copolymer lithography.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Self-Assembled Block Copolymers as a Facile Pathway to Create Functional Nanobiosensor and Nanobiomaterial Surfaces.Polymers · 2024Review
- TiOAdvanced materials (Deerfield Beach, Fla.) · 2024Article
- Lithographic Processes for the Scalable Fabrication of Micro- and Nanostructures for Biochips and Biosensors.ACS sensors · 2021Review
- A Fast and Simple Contact Printing Approach to Generate 2D Protein Nanopatterns.Frontiers in chemistry · 2018Article
- Patterning of supported gold monolayers via chemical lift-off lithography.Beilstein journal of nanotechnology · 2017Article
- Improved Glass Surface Passivation for Single-Molecule Nanoarrays.Langmuir : the ACS journal of surfaces and colloids · 2016Article
- Molecular Occupancy of Nanodot Arrays.ACS nano · 2016Article
- Hard Transparent Arrays for Polymer Pen Lithography.ACS nano · 2016Article
- Article
- Integrated micro/nanoengineered functional biomaterials for cell mechanics and mechanobiology: a materials perspective.Advanced materials (Deerfield Beach, Fla.) · 2014Review
- Large-area molecular patterning with polymer pen lithography.Nature protocols · 2013Article
- Adhesion maturation of neutrophils on nanoscopically presented platelet glycoprotein Ibα.ACS nano · 2013Article
- Surface Properties of Nanostructured Bio-Active Interfaces: Impacts of Surface Stiffness and Topography on Cell-Surface Interactions.RSC advances · 2013Article
- Delineating the pathways for the site-directed synthesis of individual nanoparticles on surfaces.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Micro- and nanoscale engineering of cell signaling.Annual review of biomedical engineering · 2013Review
Corrections and comments
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Authors and funding
4 authors.
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Abstract
The ability to control the placement of individual protein molecules on surfaces could enable advances in a wide range of areas, from the development of nanoscale biomolecular devices to fundamental studies in cell biology. Such control, however, remains a challenge in nanobiotechnology due to the limitations of current lithographic techniques. Herein we report an approach that combines scanning probe block copolymer lithography with site-selective immobilization strategies to create arrays of proteins down to the single-molecule level with arbitrary pattern control. Scanning probe block copolymer lithography was used to synthesize individual sub-10-nm single crystal gold nanoparticles that can act as scaffolds for the adsorption of functionalized alkylthiol monolayers, which facilitate the immobilization of specific proteins. The number of protein molecules that adsorb onto the nanoparticles is dependent upon particle size; when the particle size approaches the dimensions of a protein molecule, each particle can support a single protein. This was demonstrated with both gold nanoparticle and quantum dot labeling coupled with transmission electron microscopy imaging experiments. The immobilized proteins remain bioactive, as evidenced by enzymatic assays and antigen-antibody binding experiments. Importantly, this approach to generate single-biomolecule arrays is, in principle, applicable to many parallelized cantilever and cantilever-free scanning probe molecular printing methods.
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