ArticleThe Journal of biological chemistry2012
The ethylmalonyl-CoA pathway is used in place of the glyoxylate cycle by Methylobacterium extorquens AM1 during growth on acetate.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
47 citing papers in PubMed.
- LysR-type transcriptional regulator TtdR regulates both ethylene glycol and polyhydroxyalkanoate (PHA) metabolism in Pseudomonas umsongensis GO16.Applied microbiology and biotechnology · 2026Article
- Microbial diversity in Pyrenean ice caves: extreme cryogenic environments as analogues for extraterrestrial habitability.FEMS microbes · 2026Article
- Ecogenomics and functional biogeography of the Roseobacter group in the global oceans based on 653 MAGs and SAGs.Microbiome · 2025Article
- Isolation of Ultra-SmallMicroorganisms · 2025Article
- Methylobacterium extorquens PA1 utilizes multiple strategies to maintain formaldehyde homeostasis during methylotrophic growth.PLoS genetics · 2025Article
- Comparative transcription profiles ofFrontiers in microbiology · 2025Article
- Metabolic pathways to sustainability: review of purple non-sulfur bacteria potential in agri-food waste valorization.Frontiers in bioengineering and biotechnology · 2025Review
- Ethylene and epoxyethane metabolism in methanotrophic bacteria: comparative genomics and physiological studies usingMicrobial genomics · 2024Article
- From genome to evolution: investigating type II methylotrophs using a pangenomic analysis.mSystems · 2024Article
- Constructing efficient bacterial cell factories to enable one-carbon utilization based on quantitative biology: A review.Quantitative biology (Beijing, China) · 2024Review
- Pangenome analysis provides insights into the genetic diversity, metabolic versatility, and evolution of the genusMicrobiology spectrum · 2023Article
- Permafrost microbial communities and functional genes are structured by latitudinal and soil geochemical gradients.The ISME journal · 2023Article
- Asparagine Uptake: a Cellular Strategy ofApplied and environmental microbiology · 2023Article
- Comparative Genomic Analysis of aInternational journal of molecular sciences · 2023Article
- Comparative genomic analysis of Methylocystis sp. MJC1 as a platform strain for polyhydroxybutyrate biosynthesis.PloS one · 2023Article
- The Role of Microorganisms and Carbon-to-Nitrogen Ratios for Microbial Protein Production from Bioethanol.Applied and environmental microbiology · 2022Article
- Ethylmalonyl-CoA pathway involved in polyhydroxyvalerate synthesis in Candidatus Contendobacter.AMB Express · 2022Article
- Aerobic Utilization of Methanol for Microbial Growth and Production.Advances in biochemical engineering/biotechnology · 2022Article
- MinION sequencing from sea ice cryoconites leads to de novo genome reconstruction from metagenomes.Scientific reports · 2021Article
- Adaption and Degradation Strategies of Methylotrophic 1,4-Dioxane Degrading StrainInternational journal of molecular sciences · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acetyl-CoA assimilation was extensively studied in organisms harboring the glyoxylate cycle. In this study, we analyzed the metabolism of the facultative methylotroph Methylobacterium extorquens AM1, which lacks isocitrate lyase, the key enzyme in the glyoxylate cycle, during growth on acetate. MS/MS-based proteomic analysis revealed that the protein repertoire of M. extorquens AM1 grown on acetate is similar to that of cells grown on methanol and includes enzymes of the ethylmalonyl-CoA (EMC) pathway that were recently shown to operate during growth on methanol. Dynamic 13C labeling experiments indicate the presence of distinct entry points for acetate: the EMC pathway and the TCA cycle. 13C steady-state metabolic flux analysis showed that oxidation of acetyl-CoA occurs predominantly via the TCA cycle and that assimilation occurs via the EMC pathway. Furthermore, acetyl-CoA condenses with the EMC pathway product glyoxylate, resulting in malate formation. The latter, also formed by the TCA cycle, is converted to phosphoglycerate by a reaction sequence that is reversed with respect to the serine cycle. Thus, the results obtained in this study reveal the utilization of common pathways during the growth of M. extorquens AM1 on C1 and C2 compounds, but with a major redirection of flux within the central metabolism. Furthermore, our results indicate that the metabolic flux distribution is highly complex in this model methylotroph during growth on acetate and is fundamentally different from organisms using the glyoxylate cycle.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.