ArticleBMC musculoskeletal disorders2011
Cooperative effects in differentiation and proliferation between PDGF-BB and matrix derived synthetic peptides in human osteoblasts.
Article in BMC musculoskeletal disorders, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The role of peptides in bone healing and regeneration: a systematic review.BMC medicine · 2016Pooled it
- Human platelet-derived extracellular vesicle fractions modulate bone cell metabolism and biologize volume-stable β-TCP matrix in vitro.BMC medicine · 2025Article
- Low, but Not High, Pulsating Fluid Shear Stress Affects Matrix Extracellular Phosphoglycoprotein Expression, Mainly via Integrin β Subunits in Pre-Osteoblasts.Current issues in molecular biology · 2024Article
- The Osteogenic Peptide P-15 for Bone Regeneration: A Narrative Review of the Evidence for a Mechanism of Action.Bioengineering (Basel, Switzerland) · 2024Review
- Peptide-Based Biomaterials for Bone and Cartilage Regeneration.Biomedicines · 2024Review
- Self-assembled peptide hydrogel loaded with functional peptide Dentonin accelerates vascularized bone tissue regeneration in critical-size bone defects.Regenerative biomaterials · 2024Article
- Multipotential Role of Growth Factor Mimetic Peptides for Osteochondral Tissue Engineering.International journal of molecular sciences · 2022Review
- Recombinant platelet-derived growth factor-BB alleviates osteoarthritis in a rat model by decreasing chondrocyte apoptosis in vitro and in vivo.Journal of cellular and molecular medicine · 2021Article
- The Role of Growth Factors in Bioactive Coatings.Pharmaceutics · 2021Review
- Recent advances in design and applications of biomimetic self-assembled peptide hydrogels for hard tissue regeneration.Bio-design and manufacturing · 2021Review
- PDGF‑BB promotes the differentiation and proliferation of MC3T3‑E1 cells through the Src/JAK2 signaling pathway.Molecular medicine reports · 2018Article
- The use of bioactive peptides to modify materials for bone tissue repair.Regenerative biomaterials · 2017Review
- The chicken or the egg: PHEX, FGF23 and SIBLINGs unscrambled.Cell biochemistry and function · 2012Review
- Regulation of bone-renal mineral and energy metabolism: the PHEX, FGF23, DMP1, MEPE ASARM pathway.Critical reviews in eukaryotic gene expression · 2012Review
- Bioinspired synthetic peptide-based biomaterials regenerate bone through biomimicking of extracellular matrix.Journal of tissue engineeringReview
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEnhancing osteogenic capabilities of bone matrix for the treatment of fractures and segmental defects using growth factors is an active area of research. Recently, synthetic peptides like AC- 100, TP508 or p-15 corresponding to biologically active sequences of matrix proteins have been proven to stimulate bone formation. The platelet-derived growth factor (PDGF) BB has been identified as an important paracrine factor in early bone healing. We hypothesized that the combined use of PDGF-BB with synthetic peptides could result in an increase in proliferation and calcification of osteoblast-like cells.
methodsOsteoblast-like cell cultures were treated with PDGF and synthetic peptides, singly and as combinations, and compared to non-treated control cell cultures. The cultures were evaluated at days 2, 5, and 10 in terms of cell proliferation, calcification and gene expression of alkaline phosphate, collagen I and osteocalcin.
resultsExperimental findings revealed that the addition of PDGF, p-15 and TP508 and combinations of PDGF/AC-100, PDGF/p-15 and PDGF/TP508 resulted in an increase in proliferating osteoblasts, especially in the first 5 days of cultivation. Proliferation did not significantly differ between single factors and factor combinations (p > 0.05). The onset of calcification in osteoblasts occurred earlier and was more distinct compared to the corresponding control or PDGF stimulation alone. Significant difference was found for the combined use of PDGF/p-15 and PDGF/AC-100 (p < 0.05).
conclusionsOur findings indicate that PDGF exhibits cooperative effects with synthetic peptides in differentiation and proliferation. These cooperative effects cause a significant early calcification of osteoblast-like cells (p < 0.05). We suggest the combination of synthetic peptides and PDGF as a potential clinical approach for accelerating bone healing or coating osteosynthesis materials.
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