ArticleAnalytical chemistry2012
Robust analysis of the yeast proteome under 50 kDa by molecular-mass-based fractionation and top-down mass spectrometry.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- A Draft Map of E. coli Proteoforms.Analytical chemistry · 2026Article
- Fourier-transform ion cyclotron resonance mass spectrometry for characterizing proteoforms.Mass spectrometry reviews · 2022Review
- Review
- A Top-Down Proteomics Platform Coupling Serial Size Exclusion Chromatography and Fourier Transform Ion Cyclotron Resonance Mass Spectrometry.Analytical chemistry · 2019Article
- Top-Down Protein Identification using a Time-of-Flight Mass Spectrometer and Data Independent Acquisition.International journal of mass spectrometry · 2019Article
- A comprehensive pipeline for translational top-down proteomics from a single blood draw.Nature protocols · 2019Article
- Identification and Quantification of Murine Mitochondrial Proteoforms Using an Integrated Top-Down and Intact-Mass Strategy.Journal of proteome research · 2018Article
- Capillary HILIC-MS: A New Tool for Sensitive Top-Down Proteomics.Analytical chemistry · 2018Article
- Expanding Proteoform Identifications in Top-Down Proteomic Analyses by Constructing Proteoform Families.Analytical chemistry · 2018Article
- Proteoform Suite: Software for Constructing, Quantifying, and Visualizing Proteoform Families.Journal of proteome research · 2018Article
- Combined Mass Spectrometry Imaging and Top-down Microproteomics Reveals Evidence of a Hidden Proteome in Ovarian Cancer.EBioMedicine · 2017Article
- Top-Down Proteomics of Large Proteins up to 223 kDa Enabled by Serial Size Exclusion Chromatography Strategy.Analytical chemistry · 2017Article
- High-Throughput Analysis of Intact Human Proteins Using UVPD and HCD on an Orbitrap Mass Spectrometer.Journal of proteome research · 2017Article
- Heavy Sugar and Heavy Water Create Tunable Intact Protein Mass Increases for Quantitative Mass Spectrometry in Any Feed and Organism.Analytical chemistry · 2016Article
- Coupling Capillary Zone Electrophoresis to a Q Exactive HF Mass Spectrometer for Top-down Proteomics: 580 Proteoform Identifications from Yeast.Journal of proteome research · 2016Article
- Progress in Top-Down Proteomics and the Analysis of Proteoforms.Annual review of analytical chemistry (Palo Alto, Calif.) · 2016Review
- Elucidating Proteoform Families from Proteoform Intact-Mass and Lysine-Count Measurements.Journal of proteome research · 2016Article
- A Method for Label-Free, Differential Top-Down Proteomics.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Current strategies and findings in clinically relevant post-translational modification-specific proteomics.Expert review of proteomics · 2015Review
- In-line separation by capillary electrophoresis prior to analysis by top-down mass spectrometry enables sensitive characterization of protein complexes.Journal of proteome research · 2014Article
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9 authors.
Funding
Abstract
As the process of top-down mass spectrometry continues to mature, we benchmark the next installment of an improving methodology that incorporates a tube-gel electrophoresis (TGE) device to separate intact proteins by molecular mass. Top-down proteomics is accomplished in a robust fashion to yield the identification of hundreds of unique proteins, many of which correspond to multiple protein forms. The TGE platform separates 0-50 kDa proteins extracted from the yeast proteome into 12 fractions prior to automated nanocapillary LC-MS/MS in technical triplicate. The process may be completed in less than 72 h. From this study, 530 unique proteins and 1103 distinct protein species were identified and characterized, thus representing the highest coverage to date of the Saccharomyces cerevisiae proteome using top-down proteomics. The work signifies a significant step in the maturation of proteomics based on direct measurement and fragmentation of intact proteins.
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Registered trials
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