ArticlePloS one2011
Selection of stable reference genes for quantitative rt-PCR comparisons of mouse embryonic and extra-embryonic stem cells.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
52 citing papers in PubMed, 87 citations in OpenAlex.
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- Evaluation of Angelica decursiva reference genes under various stimuli for RT-qPCR data normalization.Scientific reports · 2021Article
- Selection of reliable reference genes for analysis of gene expression in the rat placenta.Molecular and cellular biochemistry · 2021Article
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- Selection and validation of experimental condition-specific reference genes for qRT-PCR in Metopolophium dirhodum (Walker) (Hemiptera: Aphididae).Scientific reports · 2020Article
- Analysis of the stability of 70 housekeeping genes during iPS reprogramming.Scientific reports · 2020Article
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- Retinoic acid signaling within pancreatic endocrine progenitors regulates mouse and human β cell specification.Development (Cambridge, England) · 2020Article
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- Reference gene selection for quantitative gene expression analysis in black soldier fly (Hermetia illucens).PloS one · 2019Article
- Flax rust infection transcriptomics reveals a transcriptional profile that may be indicative for rust Avr genes.PloS one · 2019Article
- Identification of reference genes for quantitative PCR analyses in developing mouse gonads.The Journal of veterinary medical science · 2018Article
- Identification of reference genes suitable for RT-qPCR studies of murine gastrulation and patterning.Mammalian genome : official journal of the International Mammalian Genome Society · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Isolation and culture of both embryonic and tissue specific stem cells provide an enormous opportunity to study the molecular processes driving development. To gain insight into the initial events underpinning mammalian embryogenesis, pluripotent stem cells from each of the three distinct lineages present within the preimplantation blastocyst have been derived. Embryonic (ES), trophectoderm (TS) and extraembryonic endoderm (XEN) stem cells possess the developmental potential of their founding lineages and seemingly utilize distinct epigenetic modalities to program gene expression. However, the basis for these differing cellular identities and epigenetic properties remain poorly defined.Quantitative reverse transcription-polymerase chain reaction (qPCR) is a powerful and efficient means of rapidly comparing patterns of gene expression between different developmental stages and experimental conditions. However, careful, empirical selection of appropriate reference genes is essential to accurately measuring transcriptional differences. Here we report the quantitation and evaluation of fourteen commonly used references genes between ES, TS and XEN stem cells. These included: Actb, B2m, Hsp70, Gapdh, Gusb, H2afz, Hk2, Hprt, Pgk1, Ppia, Rn7sk, Sdha, Tbp and Ywhaz. Utilizing three independent statistical analysis, we identify Pgk1, Sdha and Tbp as the most stable reference genes between each of these stem cell types. Furthermore, we identify Sdha, Tbp and Ywhaz as well as Ywhaz, Pgk1 and Hk2 as the three most stable reference genes through the in vitro differentiation of embryonic and trophectoderm stem cells respectively.Understanding the transcriptional and epigenetic regulatory mechanisms controlling cellular identity within these distinct stem cell types provides essential insight into cellular processes controlling both embryogenesis and stem cell biology. Normalizing quantitative RT-PCR measurements using the geometric mean CT values obtained for the identified mRNAs, offers a reliable method to assess differing patterns of gene expression between the three founding stem cell lineages present within the mammalian preimplantation embryo.
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