ArticleJournal of immunology (Baltimore, Md. : 1950)2011
Interaction of streptavidin-based peptide-MHC oligomers (tetramers) with cell-surface TCRs.
Article in Journal of immunology (Baltimore, Md. : 1950), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 32 citations in OpenAlex.
- Duration of Initial Viremia Modulates Functional Properties of HIV-specific T Cell Receptors.Research square · 2026Article
- Duration of Initial Viremia Modulates Functional Properties of HIV-specific T Cell Receptors.bioRxiv : the preprint server for biology · 2026Article
- Strategies and rules for tuning TCR-derived therapy.Expert reviews in molecular medicine · 2023Review
- Overcoming tumor heterogeneity by ex vivo arming of T cells using multiple bispecific antibodies.Journal for immunotherapy of cancer · 2022Article
- Potent ex vivo armed T cells using recombinant bispecific antibodies for adoptive immunotherapy with reduced cytokine release.Journal for immunotherapy of cancer · 2021Article
- TETRALEC, Artificial Tetrameric Lectins: A Tool to Screen Ligand and Pathogen Interactions.International journal of molecular sciences · 2020Article
- How the avidity of polymerase binding to the -35/-10 promoter sites affects gene expression.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Optogenetic control shows that kinetic proofreading regulates the activity of the T cell receptor.eLife · 2019Article
- Efficient magnetic enrichment of antigen-specific T cells by engineering particle properties.Biomaterials · 2018Article
- A window of opportunity for cooperativity in the T Cell Receptor.Nature communications · 2018Article
- CD4 T Cell Affinity Diversity Is Equally Maintained during Acute and Chronic Infection.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Quantitative T-cell repertoire analysis of peripheral blood mononuclear cells from lung cancer patients following long-term cancer peptide vaccination.Cancer immunology, immunotherapy : CII · 2018Article
- Subtle changes at the variable domain interface of the T-cell receptor can strongly increase affinity.The Journal of biological chemistry · 2018Article
- Comparison of T Cell Activities Mediated by Human TCRs and CARs That Use the Same Recognition Domains.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Deep Mutational Scans as a Guide to Engineering High Affinity T Cell Receptor Interactions with Peptide-bound Major Histocompatibility Complex.The Journal of biological chemistry · 2016Article
- Lower Affinity T Cells are Critical Components and Active Participants of the Immune Response.Frontiers in immunology · 2015Review
- T Cell Receptor Engineering and Analysis Using the Yeast Display Platform.Methods in molecular biology (Clifton, N.J.) · 2015Article
- Development of an IFN-γ ELISpot assay to assess varicella-zoster virus-specific cell-mediated immunity following umbilical cord blood transplantation.Journal of visualized experiments : JoVE · 2014Article
- Monovalent engagement of the BCR activates ovalbumin-specific transnuclear B cells.The Journal of experimental medicine · 2014Article
- Receptor Pre-Clustering and T cell Responses: Insights into Molecular Mechanisms.Frontiers in immunology · 2014Review
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
The binding of oligomeric peptide-MHC (pMHC) complexes to cell surface TCR can be considered to approximate TCR-pMHC interactions at cell-cell interfaces. In this study, we analyzed the equilibrium binding of streptavidin-based pMHC oligomers (tetramers) and their dissociation kinetics from CD8(pos) T cells from 2C-TCR transgenic mice and from T cell hybridomas that expressed the 2C TCR or a high-affinity mutant (m33) of this TCR. Our results show that the tetramers did not come close to saturating cell-surface TCR (binding only 10-30% of cell-surface receptors), as is generally assumed in deriving affinity values (K(D)), in part because of dissociative losses from tetramer-stained cells. Guided by a kinetic model, the oligomer dissociation rate and equilibrium constants were seen to depend not only on monovalent association and dissociation rates (k(off) and k(on)), but also on a multivalent association rate (μ) and TCR cell-surface density. Our results suggest that dissociation rates could account for the recently described surprisingly high frequency of tetramer-negative, functionally competent T cells in some T cell responses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.