ArticleNucleic acids research2012
Enhanced analysis of real-time PCR data by using a variable efficiency model: FPK-PCR.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Efficiency-Corrected Relative Quantification of qPCR Data Using LinRegPCR and a Spreadsheet-Based Workflow.Bio-protocol · 2026Article
- Analysis of qPCR Data: From PCR Efficiency to Absolute Target Quantity.International journal of molecular sciences · 2025Review
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- Introducing the fBMC bioinformatics · 2024Article
- Wide reference databases for typing Trypanosoma cruzi based on amplicon sequencing of the minicircle hypervariable region.PLoS neglected tropical diseases · 2023Article
- Reducing Bias and Quantifying Uncertainty in Fluorescence Produced by PCR.Bulletin of mathematical biology · 2023Article
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- Binding, brightness, or noise? Extracting temperature-dependent properties of dye bound to DNA.Biophysical journal · 2023Article
- qPCRtools: An R package for qPCR data processing and visualization.Frontiers in genetics · 2022Article
- Evaluation validation of a qPCR curve analysis method and conventional approaches.BMC genomics · 2021Article
- Saliva is Comparable to Nasopharyngeal Swabs for Molecular Detection of SARS-CoV-2.Microbiology spectrum · 2021Article
- Web-based LinRegPCR: application for the visualization and analysis of (RT)-qPCR amplification and melting data.BMC bioinformatics · 2021Article
- Estimating Real-Time qPCR Amplification Efficiency from Single-Reaction Data.Life (Basel, Switzerland) · 2021Article
- Nucleic Acid Testing of SARS-CoV-2.International journal of molecular sciences · 2021Review
- Autoregressive modeling and diagnostics for qPCR amplification.Bioinformatics (Oxford, England) · 2021Article
- Affine analysis for quantitative PCR measurements.Analytical and bioanalytical chemistry · 2020Article
- Pairwise efficiency: a new mathematical approach to qPCR data analysis increases the precision of the calibration curve assay.BMC bioinformatics · 2019Article
- qPCR data analysis: Better results through iconoclasm.Biomolecular detection and quantification · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Current methodology in real-time Polymerase chain reaction (PCR) analysis performs well provided PCR efficiency remains constant over reactions. Yet, small changes in efficiency can lead to large quantification errors. Particularly in biological samples, the possible presence of inhibitors forms a challenge. We present a new approach to single reaction efficiency calculation, called Full Process Kinetics-PCR (FPK-PCR). It combines a kinetically more realistic model with flexible adaptation to the full range of data. By reconstructing the entire chain of cycle efficiencies, rather than restricting the focus on a 'window of application', one extracts additional information and loses a level of arbitrariness. The maximal efficiency estimates returned by the model are comparable in accuracy and precision to both the golden standard of serial dilution and other single reaction efficiency methods. The cycle-to-cycle changes in efficiency, as described by the FPK-PCR procedure, stay considerably closer to the data than those from other S-shaped models. The assessment of individual cycle efficiencies returns more information than other single efficiency methods. It allows in-depth interpretation of real-time PCR data and reconstruction of the fluorescence data, providing quality control. Finally, by implementing a global efficiency model, reproducibility is improved as the selection of a window of application is avoided.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.