ArticleThe Journal of biological chemistry2012
Efflux permease CgAcr3-1 of Corynebacterium glutamicum is an arsenite-specific antiporter.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 40 citations in OpenAlex.
- Arsenic-sensing domain controls ACR3 transporter trafficking in Marchantia polymorpha.The Plant cell · 2026Article
- Arsenic efflux mechanisms in ectomycorrhizal mushrooms Hebeloma bulbiferum and Hebeloma sinapizans.Applied microbiology and biotechnology · 2026Article
- Adaptation to metal(loid)s in strain Mucilaginibacter rubeus P2 involves novel arsenic resistance genes and mechanisms.Journal of hazardous materials · 2024Article
- The ArsQ permease and transport of the antibiotic arsinothricin.Molecular microbiology · 2023Article
- Biochemical, genomic and structural characteristics of the Acr3 pump inFrontiers in microbiology · 2022Article
- ArsV and ArsW provide synergistic resistance to the antibiotic methylarsenite.Environmental microbiology · 2021Article
- Identification of the Biosynthetic Gene Cluster for the Organoarsenical Antibiotic Arsinothricin.Microbiology spectrum · 2021Article
- Comamonas testosteroni antA encodes an antimonite-translocating P-type ATPase.The Science of the total environment · 2021Article
- A Novel Mobile Element ICEFrontiers in microbiology · 2020Article
- Pathways of arsenic uptake and efflux.Environment international · 2019Review
- Biostimulation potentials of corn steep liquor in enhanced hydrocarbon degradation in chronically polluted soil.3 Biotech · 2019Article
- Arsenic metabolism in high altitude modern stromatolites revealed by metagenomic analysis.Scientific reports · 2017Article
- Metagenomic insights into effects of spent engine oil perturbation on the microbial community composition and function in a tropical agricultural soil.Environmental science and pollution research international · 2017Article
- Synergistic interaction of glyceraldehydes-3-phosphate dehydrogenase and ArsJ, a novel organoarsenical efflux permease, confers arsenate resistance.Molecular microbiology · 2016Article
- ArsP: a methylarsenite efflux permease.Molecular microbiology · 2015Article
- Pathways of arsenic uptake and efflux.Current topics in membranes · 2012Article
- Arsenic and antimony transporters in eukaryotes.International journal of molecular sciences · 2012Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
Abstract
Resistance to arsenite (As(III)) by cells is generally accomplished by arsenite efflux permeases from Acr3 or ArsB unrelated families. We analyzed the function of three Acr3 proteins from Corynebacterium glutamicum, CgAcr3-1, CgAcr3-2, and CgAcr3-3. CgAcr3-1 conferred the highest level of As(III) resistance and accumulation in vivo. CgAcr3-1 was also the most active when everted membranes vesicles from Escherichia coli or C. glutamicum mutants were assayed for efflux with different energy sources. As(III) and antimonite (Sb(III)) resistance and accumulation studies using E. coli or C. glutamicum arsenite permease mutants clearly show that CgAcr3-1 is specific for As(III). In everted membrane vesicles expressing CgAcr3-1, dissipation of either the membrane potential or the pH gradient of the proton motive force did not prevent As(III) uptake, whereas dissipation of both components eliminated uptake. Further, a mutagenesis study of CgAcr3-1 suggested that a conserved cysteine and glutamate are involved in active transport. Therefore, we propose that CgAcr3-1 is an antiporter that catalyzes arsenite-proton exchange with residues Cys129 and Glu305 involved in efflux.
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