ArticleThe protein journal2012
The effects of temperature and pH on secondary structure and antioxidant activity of Crocodylus siamensis hemoglobin.
Article in The protein journal, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 39 citations in OpenAlex.
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- Evaluation of TILI-2 as an Anti-Tyrosinase, Anti-Oxidative Agent and Its Role in Preventing Melanogenesis Using a Proteomics Approach.Molecules (Basel, Switzerland) · 2022Article
- Effect of Supercritical Extract from Black Poplar and Basket Willow on the Quality of Natural and Probiotic Drinkable Yogurt.Animals : an open access journal from MDPI · 2021Article
- Hemoglobin structure at higher levels of hemoglobin A1C in type 2 diabetes and associated complications.Chinese medical journal · 2020Article
- Protective Effect of Crocodile Hemoglobin and Whole Blood Against Hydrogen Peroxide-Induced Oxidative Damage in Human Lung Fibroblasts (MRC-5) and Inflammation in Mice.Inflammation · 2017Article
- Immunological properties of oxygen-transport proteins: hemoglobin, hemocyanin and hemerythrin.Cellular and molecular life sciences : CMLS · 2017Review
- The impact of the HbA1c level of type 2 diabetics on the structure of haemoglobin.Scientific reports · 2016Article
- Cloning, Expression, and Characterization of Siamese Crocodile (Crocodylus siamensis) Hemoglobin from Escherichia coli and Pichia pastoris.The protein journal · 2016Article
- An investigation of antioxidant and anti-inflammatory activities from blood components of Crocodile (Crocodylus siamensis).The protein journal · 2014Article
- Purification, characterization, and crystallization of Crocodylus siamensis hemoglobin.The protein journal · 2014Article
- Antimicrobial peptides in reptiles.Pharmaceuticals (Basel, Switzerland) · 2014Article
- Purification and characterization of antioxidant peptides from leukocyte extract of Crocodylus siamensis.The protein journal · 2014Article
- Molecular cloning and expression of α-globin and β-globin genes from crocodile (Crocodylus siamensis).The protein journal · 2013Article
- Complete amino acid sequence of globin chains and biological activity of fragmented crocodile hemoglobin (Crocodylus siamensis).The protein journal · 2012Article
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Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Crocodylus siamensis hemoglobin (cHb) was purified by gel filtration chromatography and visualized by SDS-PAGE. Effects of temperature and pH on secondary structure and conformation changes of cHb were studied using circular dichroism spectropolarimeter and fourier transform infrared spectrophotometer. The secondary structure of intact cHb was mainly α-helices. cHb was not heat stable when heated at 65 °C and cooled down to original temperature, indicating the irreversible unfolding process. The stability of cHb at different pH ranging from 2.5 to 10.5 was determined. The maximum value of the α-helix content was found at pH 3.5 and tended to decrease at strong acid and strong base. The antioxidant activities of heat treated cHb and cHb in solution with pH range 2.5 to 10.5 were tested by DPPH radical scavenging assay. cHb at pH 4.5, having highest β-turn structure, showed highest radical scavenging activity. In contrast to pH, heat had no effect on antioxidant activity of cHb.
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