ArticleApplied and environmental microbiology2012
Role of IncP-1β plasmids pWDL7::rfp and pNB8c in chloroaniline catabolism as determined by genomic and functional analyses.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
- Not So Mosaic After All? The Core Genomes of IncP-1 Plasmids Evolve Predominantly Through Vertical Transmission.bioRxiv : the preprint server for biology · 2025Article
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- Comparative Genomics Suggests Mechanisms of Genetic Adaptation toward the Catabolism of the Phenylurea Herbicide Linuron in Variovorax.Genome biology and evolution · 2020Article
- Molecular processes underlying synergistic linuron mineralization in a triple-species bacterial consortium biofilm revealed by differential transcriptomics.MicrobiologyOpen · 2018Article
- Comparison of the complete genome sequences of four γ-hexachlorocyclohexane-degrading bacterial strains: insights into the evolution of bacteria able to degrade a recalcitrant man-made pesticide.DNA research : an international journal for rapid publication of reports on genes and genomes · 2016Article
- Functional Redundancy of Linuron Degradation in Microbial Communities in Agricultural Soil and Biopurification Systems.Applied and environmental microbiology · 2016Article
- Comparison of Four Comamonas Catabolic Plasmids Reveals the Evolution of pBHB To Catabolize Haloaromatics.Applied and environmental microbiology · 2015Article
- Plasmid Detection, Characterization, and Ecology.Microbiology spectrum · 2015Review
- Bacterial degradation of monocyclic aromatic amines.Frontiers in microbiology · 2015Review
- Shifts in abundance and diversity of mobile genetic elements after the introduction of diverse pesticides into an on-farm biopurification system over the course of a year.Applied and environmental microbiology · 2014Article
- Borrelia chilensis, a new member of the Borrelia burgdorferi sensu lato complex that extends the range of this genospecies in the Southern Hemisphere.Environmental microbiology · 2014Article
- Article
- Function of a glutamine synthetase-like protein in bacterial aniline oxidation via γ-glutamylanilide.Journal of bacteriology · 2013Article
- Widespread dissemination of class 1 integron components in soils and related ecosystems as revealed by cultivation-independent analysis.Frontiers in microbiology · 2013Article
- Inferring the evolutionary history of IncP-1 plasmids despite incongruence among backbone gene trees.Molecular biology and evolution · 2013Article
- Permanent draft genome sequence of Comamonas testosteroni KF-1.Standards in genomic sciences · 2013Article
- Roles of long and short replication initiation proteins in the fate of IncP-1 plasmids.Journal of bacteriology · 2012Article
- Frequency of disturbance alters diversity, function, and underlying assembly mechanisms of complex bacterial communities.NPJ biofilms and microbiomesArticle
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
Abstract
Broad-host-range catabolic plasmids play an important role in bacterial degradation of man-made compounds. To gain insight into the role of these plasmids in chloroaniline degradation, we determined the first complete nucleotide sequences of an IncP-1 chloroaniline degradation plasmid, pWDL7::rfp and its close relative pNB8c, as well as the expression pattern, function, and bioaugmentation potential of the putative 3-chloroaniline (3-CA) oxidation genes. Based on phylogenetic analysis of backbone proteins, both plasmids are members of a distinct clade within the IncP-1β subgroup. The plasmids are almost identical, but whereas pWDL7::rfp carries a duplicate inverted catabolic transposon, Tn6063, containing a putative 3-CA oxidation gene cluster, dcaQTA1A2BR, pNB8c contains only a single copy of the transposon. No genes for an aromatic ring cleavage pathway were detected on either plasmid, suggesting that only the upper 3-CA degradation pathway was present. The dcaA1A2B gene products expressed from a high-copy-number vector were shown to convert 3-CA to 4-chlorocatechol in Escherichia coli. Slight differences in the dca promoter region between the plasmids and lack of induction of transcription of the pNB8c dca genes by 3-CA may explain previous findings that pNB8C does not confer 3-CA transformation. Bioaugmentation of activated sludge with pWDL7::rfp accelerated removal of 3-CA, but only in the presence of an additional carbon source. Successful bioaugmentation requires complementation of the upper pathway genes with chlorocatechol cleavage genes in indigenous bacteria. The genome sequences of these plasmids thus help explain the molecular basis of their catabolic activities.
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