ArticleApplied and environmental microbiology2012
Responses of methanogen mcrA genes and their transcripts to an alternate dry/wet cycle of paddy field soil.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers, 1 of them a synthesis that pooled it.
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Who cites it
37 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Effects of Water and Fertilizer Management Practices on Methane Emissions from Paddy Soils: Synthesis and Perspective.International journal of environmental research and public health · 2022Pooled it
- The balance between microbial arsenic methylation and demethylation in paddy soils underpins global arsenic risk and straighthead disease in rice.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Long-Term Grazing and Nitrogen Management Impacted Methane Emission Potential and Soil Microbial Community in Grazing Pastures.Environment & health (Washington, D.C.) · 2025Article
- Article
- Fertilization and Global Warming Impact on Paddy CHInternational journal of environmental research and public health · 2023Article
- Sink or Source: Alternative Roles of Glacier Foreland Meadow Soils in Methane Emission Is Regulated by Glacier Melting on the Tibetan Plateau.Frontiers in microbiology · 2022Article
- Disentangling Responses of the Subsurface Microbiome to Wetland Status and Implications for Indicating Ecosystem Functions.Microorganisms · 2021Article
- Methane Production in Soil Environments-Anaerobic Biogeochemistry and Microbial Life between Flooding and Desiccation.Microorganisms · 2020Review
- Microbial functional diversity: From concepts to applications.Ecology and evolution · 2019Review
- Three-Source Partitioning of Methane Emissions from Paddy Soil: Linkage to Methanogenic Community Structure.International journal of molecular sciences · 2019Article
- Cryptic CHThe ISME journal · 2019Article
- NanoFeFrontiers in microbiology · 2019Article
- Metatranscriptomics reveals a differential temperature effect on the structural and functional organization of the anaerobic food web in rice field soil.Microbiome · 2018Article
- Transcription of mcrA Gene Decreases Upon Prolonged Non-flooding Period in a Methanogenic Archaeal Community of a Paddy-Upland Rotational Field Soil.Microbial ecology · 2018Article
- Cofactor F430 as a biomarker for methanogenic activity: application to an anaerobic bioreactor system.Applied microbiology and biotechnology · 2018Article
- Reduction in Methane Emissions From Acidified Dairy Slurry Is Related to Inhibition ofFrontiers in microbiology · 2018Article
- Stimulatory Effect of Magnetite Nanoparticles on a Highly Enriched Butyrate-Oxidizing Consortium.Frontiers in microbiology · 2018Article
- Copper Pollution Increases the Resistance of Soil Archaeal Community to Changes in Water Regime.Microbial ecology · 2017Article
- Metatranscriptomic Evidence for Direct Interspecies Electron Transfer between Geobacter and Methanothrix Species in Methanogenic Rice Paddy Soils.Applied and environmental microbiology · 2017Article
- Methane Emissions and Microbial Communities as Influenced by Dual Cropping of Azolla along with Early Rice.Scientific reports · 2017Article
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Authors and funding
3 authors.
Funding
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Abstract
Intermittent drainage can substantially reduce methane emission from rice fields, but the microbial mechanisms remain poorly understood. In the present study, we determined the rates of methane production and emission, the dynamics of ferric iron and sulfate, and the abundance of methanogen mcrA genes (encoding the alpha subunit of methyl coenzyme M reductase) and their transcripts in response to alternate dry/wet cycles in paddy field soil. We found that intermittent drainage did not affect the growth of rice plants but significantly reduced the rates of both methane production and emission. The dry/wet cycles also resulted in shifts of soil redox conditions, increasing the concentrations of ferric iron and sulfate in the soil. Quantitative PCR analysis revealed that both mcrA gene copies and mcrA transcripts significantly decreased after dry/wet alternation compared to continuous flooding. Correlation and regression analyses showed that the abundance of mcrA genes and transcripts positively correlated with methane production potential and soil water content and negatively correlated with the concentrations of ferric iron and sulfate in the soil. However, the transcription of mcrA genes was reduced to a greater extent than the abundance of mcrA genes, resulting in very low mcrA transcript/gene ratios after intermittent drainage. Furthermore, terminal restriction fragment length polymorphism analysis revealed that the composition of methanogenic community remained stable under dry/wet cycles, whereas that of metabolically active methanogens strongly changed. Collectively, our study demonstrated a stronger effect of intermittent drainage on the abundance of mcrA transcripts than of mcrA genes in rice field soil.
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