ArticleJournal of lipid research2012
Enzymatic measurement of phosphatidylserine in cultured cells.
Article in Journal of lipid research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 33 citations in OpenAlex.
- Synthetic Lipid Biology.Chemical reviews · 2025Review
- Phospholipid biomarkers of coronary heart disease.Journal of pharmaceutical health care and sciences · 2024Review
- Short Antimicrobial Peptide Derived from the Venom Gland Transcriptome ofAntibiotics (Basel, Switzerland) · 2023Article
- Current trends in luminescence-based assessment of apoptosis.RSC advances · 2023Review
- The mechanical, optical, and thermal properties of graphene influencing its pre-clinical use in treating neurological diseases.Frontiers in neuroscience · 2023Review
- Alterations in cellular and organellar phospholipid compositions of HepG2 cells during cell growth.Scientific reports · 2021Article
- Protocols for Enzymatic Fluorometric Assays to Quantify Phospholipid Classes.International journal of molecular sciences · 2020Review
- The effect of ageing on the resolution of inflammation.Ageing research reviews · 2020Review
- Lipid class composition of membrane and raft fractions from brains of individuals with Alzheimer's disease.Biochemistry and biophysics reports · 2019Article
- Enzymatic fluorometric assays for quantifying all major phospholipid classes in cells and intracellular organelles.Scientific reports · 2019Article
- NaAutism research : official journal of the International Society for Autism Research · 2018Article
- Variation in human cancer cell external phosphatidylserine is regulated by flippase activity and intracellular calcium.Oncotarget · 2015Article
- Isolation of Endoplasmic Reticulum, Mitochondria, and Mitochondria-Associated Membrane and Detergent Resistant Membrane Fractions from Transfected Cells and from Human Cytomegalovirus-Infected Primary Fibroblasts.Current protocols in cell biology · 2015Article
- Host Cell Plasma Membrane Phosphatidylserine Regulates the Assembly and Budding of Ebola Virus.Journal of virology · 2015Article
- Enzymatic measurement of phosphatidylglycerol and cardiolipin in cultured cells and mitochondria.Scientific reports · 2015Article
- Gain-of-function mutations in the phosphatidylserine synthase 1 (PTDSS1) gene cause Lenz-Majewski syndrome.Nature genetics · 2014Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphatidylserine (PS) is a quantitatively minor membrane phospholipid involved in diverse cellular functions. In this study, we developed a new fluorometric method for measuring PS using combinations of specific enzymes and Amplex Red. The calibration curve for PS measurement was linear and hyperbolic at low (0-50 µM) and high (50-1000 µM) concentrations, respectively, and the detection limit was 5 µM (50 pmol in the reaction mixture). This assay quantified PS regardless of the chain length and the number of double bonds. We applied this new method to the determination of PS content in HEK293 cells, which was validated by a recovery study and comparison with TLC-phosphorus assay. We showed that the PS content was high in sparse cells. The overexpression of PS synthase 1 elevated not only the cellular PS content but also the phosphatidylcholine (PC) and phosphatidylethanolamine (PE) contents, suggesting the conversion of PS into PE and the enhancement of PC production. This new assay for PS measurement is simple, specific, sensitive, and high throughput, and it will be useful to clarify the metabolism and biological functions of PS.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.