ArticleBMC veterinary research2011
Serological testing of cattle experimentally infected with Mycoplasma mycoides subsp. mycoides Small Colony using four different tests reveals a variety of seroconversion patterns.
Article in BMC veterinary research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- A systematic review and meta-analysis of contagious bovine pleuropneumonia in Ethiopian cattle.Veterinary and animal science · 2024Review
- Contagious Bovine and Caprine Pleuropneumonia: a research community's recommendations for the development of better vaccines.NPJ vaccines · 2020Review
- Methodological aspects of serosurveillance in resource-poor settings.Veterinary record open · 2018Article
- Seroprevalence and Distribution of Contagious Bovine Pleuropneumonia in Ethiopia: Update and Critical Analysis of 20 Years (1996-2016) Reports.Frontiers in veterinary science · 2017Review
- Development of a Novel Cocktail Enzyme-Linked Immunosorbent Assay and a Field-Applicable Lateral-Flow Rapid Test for Diagnosis of Contagious Bovine Pleuropneumonia.Journal of clinical microbiology · 2016Article
- Contagious bovine pleuropneumonia: Seroprevalence and risk factors in Western Oromia, Ethiopia.The Onderstepoort journal of veterinary research · 2016Article
- Sero-positivity and associated risk factors for contagious bovine pleuropneumonia under two cattle production systems in North Central Nigeria.Tropical animal health and production · 2016Article
- Antibody response to Mycoplasma capricolum subsp. capripneumoniae bacterium in small holder dairy goats in Tanzania.Tropical animal health and production · 2013Article
- Isothermal loop-mediated amplification (LAMP) for diagnosis of contagious bovine pleuro-pneumonia.BMC veterinary research · 2013Article
- Private sector involvement in the control of contagious bovine pleuropneumonia (CBPP) in the Kazungula district of Zambia benefitted the community and the control strategy.Tropical animal health and production · 2013Review
- Seroprevalence of contagious bovine pleuropneumonia at export quarantine centers in and around Adama, Ethiopia.Tropical animal health and production · 2013Article
- Performance evaluation of two serological tests for contagious bovine pleuropneumonia (CBPP) detection in an enzootic area using a Bayesian framework.Tropical animal health and production · 2012Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTo study the specific antibody response to infection with Mycoplasma mycoides subsp. mycoides Small Colony (MmmSC), the agent of Contagious Bovine Pleuropneumonia (CBPP), we examined three panels of sera collected during three experimental infection trials in African cattle. The methods used included an in-house complement fixation test (CFT), a commercially available CFT, a competitive antibody ELISA (cELISA) and the immunoblotting test (IBT). In addition, lung tissue samples were examined by culture.
resultsA total of 89% (51/59) of all experimentally infected animals tested positive on at least one of the serological tests throughout the trial. The specific antibody titres to the MmmSC infection became positive first by CFT (6 to 9 days post infection [dpi]), followed by IBT (9 to 13 dpi) and cELISA (13 to 16 dpi). Individual animals were found to display remarkably distinct seroconversion patterns, which allowed their classification into i) early high responders, ii) late high responders, and iii) low responders. In accordance with other studies, none of the present serological tests was capable of detecting all CBPP infected animals.
conclusionComparison of the assays' performance in terms of sensitivity and specificity raises serious questions as to their reliability for identification of infected individuals in the field. In view of these limitations, a combination of CFT and cELISA can markedly improve CBPP diagnosis at single-animal level.
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