ArticleBiophysical journal2011
Single cell analysis of ligand binding and complex formation of interleukin-4 receptor subunits.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.
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Who cites it
15 citing papers in PubMed, 1 synthesis or guideline pooled it, 34 citations in OpenAlex.
- Biomarkers of rheumatoid arthritis-associated interstitial lung disease: a systematic review and meta-analysis.Frontiers in immunology · 2024Pooled it
- In Situ Identification of Both IL-4 and IL-10 Cytokine-Receptor Interactions during Tissue Regeneration.Cells · 2023Article
- Molecular and cellular factors determining the functional pleiotropy of cytokines.The FEBS journal · 2023Review
- Insights into receptor structure and dynamics at the surface of living cells.Nature communications · 2023Article
- Article
- Spatial considerations in the resolution of inflammation: Elucidating leukocyte interactions via an experimentally-calibrated agent-based model.PLoS computational biology · 2020Article
- Mechanisms and Points of Control in the Spread of Inflammation: A Mathematical Investigation.Bulletin of mathematical biology · 2020Article
- Diffusion of Single-Pass Transmembrane Receptors: From the Plasma Membrane into Giant Liposomes.The Journal of membrane biology · 2017Article
- Dopamine Receptor Signaling in MIN6 β-Cells Revealed by Fluorescence Fluctuation Spectroscopy.Biophysical journal · 2016Article
- Membrane targeting of the Spir·formin actin nucleator complex requires a sequential handshake of polar interactions.The Journal of biological chemistry · 2015Article
- Dynamics and interaction of interleukin-4 receptor subunits in living cells.Biophysical journal · 2014Article
- Gathering support for critical mass: interleukin 4 receptor signaling requires clustering in endosomes.Biophysical journal · 2014Article
- Multifarious determinants of cytokine receptor signaling specificity.Advances in immunology · 2014Review
- Loss-of-function mutations in the IL-21 receptor gene cause a primary immunodeficiency syndrome.The Journal of experimental medicine · 2013Article
- Competition for IL-2 between Regulatory and Effector T Cells to Chisel Immune Responses.Frontiers in immunology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interleukin-4 (IL-4) is an important class I cytokine involved in adaptive immunity. IL-4 binds with high affinity to the single-pass transmembrane receptor IL-4Rα. Subsequently, IL-4Rα/IL-4 is believed to engage a second receptor chain, either IL-2Rγ or IL-13Rα1, to form type I or II receptor complexes, respectively. This ternary complex formation then triggers downstream signaling via intracellular Janus kinases bound to the cytoplasmic receptor tails. Here, we study the successive steps of complex formation at the single cell level with confocal fluorescence imaging and correlation spectroscopy. We characterize binding and signaling of fluorescently labeled IL-4 by flow cytometry of IL-4-dependent BaF3 cells. The affinity to ectopically expressed IL-4Rα was then measured by single-color fluorescence correlation spectroscopy in adherent HEK293T cells that express the components of the type II IL-4R but not type I. Finally, IL-4-induced complex formation was tested by dual-color fluorescence cross-correlation spectroscopy. The data provide evidence for codiffusion of IL-4-A647 bound IL-4Rα and the type II subunit IL-13Rα1 fused to enhanced green fluorescent protein, whereas type I complexes containing IL-2Rγ and JAK3 were not detected at the cell surface. This behavior may reflect hitherto undefined differences in the mode of receptor activation between type I (lymphoid) and type II (epithelial) receptor expressing cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.