ArticleBiophysical journal2011
Native ultrastructure of the red cell cytoskeleton by cryo-electron tomography.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed, 109 citations in OpenAlex.
- Influence of leukocyte adhesion on partitioning of healthy and diabetic red blood cells at vascular bifurcations.Biophysical journal · 2026Article
- Dynamic mechanisms for membrane skeleton transitions.Journal of cell science · 2025Article
- Mechanical characterization of spectrin at the molecular level.Scientific reports · 2024Article
- Advancements and Practical Considerations for Biophysical Research: Navigating the Challenges and Future of Super-resolution Microscopy.Chemical & biomedical imaging · 2024Review
- Dynamic mechanisms for membrane skeleton transitions.bioRxiv : the preprint server for biology · 2024Article
- Energy Dissipation in the Human Red Cell Membrane.Biomolecules · 2023Article
- Lingering Dynamics of Type 2 Diabetes Mellitus Red Blood Cells in Retinal Arteriolar Bifurcations.Journal of functional biomaterials · 2022Article
- RBC membrane biomechanics and Plasmodium falciparum invasion: probing beyond ligand-receptor interactions.Trends in parasitology · 2022Review
- In-depth biological analysis of alteration in Plasmodium knowlesi-infected red blood cells using a noninvasive optical imaging technique.Parasites & vectors · 2022Article
- Nanoscale dynamics of actin filaments in the red blood cell membrane skeleton.Molecular biology of the cell · 2022Article
- Multimodal imaging reveals membrane skeleton reorganisation during reticulocyte maturation and differences in dimple and rim regions of mature erythrocytes.Journal of structural biology: X · 2022Article
- Quantifying the influences of radiation therapy on deformability of human red blood cells by dual-beam optical tweezers.RSC advances · 2021Article
- Constitutive Model of Erythrocyte Membranes with Distributions of Spectrin Orientations and Lengths.Biophysical journal · 2020Article
- Human erythrocytes: cytoskeleton and its origin.Cellular and molecular life sciences : CMLS · 2020Review
- Multimodal analysis of Plasmodium knowlesi-infected erythrocytes reveals large invaginations, swelling of the host cell, and rheological defects.Cellular microbiology · 2019Article
- The knob protein KAHRP assembles into a ring-shaped structure that underpins virulence complex assembly.PLoS pathogens · 2019Article
- Effects of Polyphenol Compounds and Nitrogen Oxide Donors on Lipid Oxidation, Membrane-Skeletal Proteins, and Erythrocyte Structure under Hypoxia.BioMed research international · 2019Article
- Shape changes of erythrocytes during blood clot contraction and the structure of polyhedrocytes.Scientific reports · 2018Article
- Squeezing for Life - Properties of Red Blood Cell Deformability.Frontiers in physiology · 2018Review
- Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Erythrocytes possess a spectrin-based cytoskeleton that provides elasticity and mechanical stability necessary to survive the shear forces within the microvasculature. The architecture of this membrane skeleton and the nature of its intermolecular contacts determine the mechanical properties of the skeleton and confer the characteristic biconcave shape of red cells. We have used cryo-electron tomography to evaluate the three-dimensional topology in intact, unexpanded membrane skeletons from mouse erythrocytes frozen in physiological buffer. The tomograms reveal a complex network of spectrin filaments converging at actin-based nodes and a gradual decrease in both the density and the thickness of the network from the center to the edge of the cell. The average contour length of spectrin filaments connecting junctional complexes is 46 ± 15 nm, indicating that the spectrin heterotetramer in the native membrane skeleton is a fraction of its fully extended length (∼190 nm). Higher-order oligomers of spectrin were prevalent, with hexamers and octamers seen between virtually every junctional complex in the network. Based on comparisons with expanded skeletons, we propose that the oligomeric state of spectrin is in a dynamic equilibrium that facilitates remodeling of the network as the cell changes shape in response to shear stress.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.