Evidence map›Paper›PMID 22096114›Full record

ArticleMolecular & cellular proteomics : MCP2012

Proteomic analysis of human saliva from lung cancer patients using two-dimensional difference gel electrophoresis and mass spectrometry.

Hua Xiao, Lei Zhang, Hui Zhou, Jay M Lee, Edward B Garon, David T W Wong

Open access · hybridAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular & cellular proteomics : MCP, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 84 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
84citing papers in PubMed, 3 pooled it
9.5field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

84 citing papers in PubMed, 3 syntheses or guidelines pooled it, 191 citations in OpenAlex.

  1. Pooled it
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  4. Noninvasive saliva-based EGFR gene mutation detection in patients with lung cancer.American journal of respiratory and critical care medicine · 2014
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24 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Hua XiaoDental Research Institute, University of California, Los Angeles, Los Angeles, California 90095, USA. xiaohuacn@gmail.com
Lei Zhang
Hui Zhou
Jay M Lee
Edward B Garon
David T W Wong
Samueli Institute · USUCLA Jonsson Comprehensive Cancer CenterUniversity of California, Los Angeles · US

Funding

Human Salivary ProteomeU01DE016275 · NIDCR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WONG, DAVID T · 2004 to 2007
$5.5M
Inhibiting EGFR and ER pathways in NSCLCK23CA149079 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GARON, EDWARD B · 2011 to 2015
$826k
Collection, Stabilization and Storage of Saliva Samples for Cancer ResearchR21CA126733 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI WONG, DAVID T · 2008 to 2009
$381k
NCI NIH HHS K23 CA149079NCI NIH HHS R21-CA0126733NCI NIH HHS R21 CA126733NIDCR NIH HHS U01 DE016275NIDCR NIH HHS U01-DE16275
6 · The paper itself

Abstract

Lung cancer is often asymptomatic or causes only nonspecific symptoms in its early stages. Early detection represents one of the most promising approaches to reduce the growing lung cancer burden. Human saliva is an attractive diagnostic fluid because its collection is less invasive than that of tissue or blood. Profiling of proteins in saliva over the course of disease progression could reveal potential biomarkers indicative of oral or systematic diseases, which may be used extensively in future medical diagnostics. There were 72 subjects enrolled in this study for saliva sample collection according to the approved protocol. Two-dimensional difference gel electrophoresis combined with MS was the platform for salivary proteome separation, quantification, and identification from two pooled samples. Candidate proteomic biomarkers were verified and prevalidated by using immunoassay methods. There were 16 candidate protein biomarkers discovered by two-dimensional difference gel electrophoresis and MS. Three proteins were further verified in the discovery sample set, prevalidation sample set, and lung cancer cell lines. The discriminatory power of these candidate biomarkers in lung cancer patients and healthy control subjects can reach 88.5% sensitivity and 92.3% specificity with AUC = 0.90. This preliminary data report demonstrates that proteomic biomarkers are present in human saliva when people develop lung cancer. The discriminatory power of these candidate biomarkers indicate that a simple saliva test might be established for lung cancer clinical screening and detection.

Indexed as

AgedAged, 80 and overBiomarkers, TumorBlotting, WesternCarcinoma, Non-Small-Cell LungCase-Control StudiesCells, CulturedEarly Detection of CancerEnzyme-Linked Immunosorbent AssayFemaleHumansLungLung NeoplasmsMaleMiddle AgedProteomeBiomarkers, TumorProteome

Identifiers

PMID22096114
PMCPMC3277759
OpenAlexW2166794462

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.