ArticleDevelopment (Cambridge, England)2012
Gβγ signaling controls the polarization of zebrafish primordial germ cells by regulating Rac activity.
Article in Development (Cambridge, England), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 26 citations in OpenAlex.
- The journey of a generation: advances and promises in the study of primordial germ cell migration.Development (Cambridge, England) · 2024Article
- Article
- Review
- Maternal miR-202-5p is required for zebrafish primordial germ cell migration by protecting small GTPase Cdc42.Journal of molecular cell biology · 2020Article
- The mevalonate pathway is a crucial regulator of tendon cell specification.Development (Cambridge, England) · 2020Article
- A Genetically Encoded Biosensor Strategy for Quantifying Non-muscle Myosin II Phosphorylation Dynamics in Living Cells and Organisms.Cell reports · 2018Article
- Gβ1 is required for neutrophil migration in zebrafish.Developmental biology · 2017Article
- Methods to study maternal regulation of germ cell specification in zebrafish.Methods in cell biology · 2016Article
- Daple is a novel non-receptor GEF required for trimeric G protein activation in Wnt signaling.eLife · 2015Article
- Chemokine-guided cell migration and motility in zebrafish development.The EMBO journal · 2015Review
- The dynamics of Rho GTPase signaling and implications for targeting cancer and the tumor microenvironment.Small GTPases · 2015Review
- Transducin duplicates in the zebrafish retina and pineal complex: differential specialisation after the teleost tetraploidisation.PloS one · 2015Article
- The Rac-FRET mouse reveals tight spatiotemporal control of Rac activity in primary cells and tissues.Cell reports · 2014Article
- Gβ1 controls collective cell migration by regulating the protrusive activity of leader cells in the posterior lateral line primordium.Developmental biology · 2014Article
- Molecular characteristics of malignant ovarian germ cell tumors and comparison with testicular counterparts: implications for pathogenesis.Endocrine reviews · 2013Review
- Nodal signaling regulates endodermal cell motility and actin dynamics via Rac1 and Prex1.The Journal of cell biology · 2012Article
- Neutrophil reverse migration becomes transparent with zebrafish.Advances in hematology · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
During development, primordial germ cells (PGCs) migrate from the sites of their specification towards the region in which the future gonad develops. This cell migration requires polarization of PGCs and their responsiveness to external guidance cues. In zebrafish, the directed migration and polarization of PGCs are regulated independently, by the chemokine Cxcl12a and the Rho GTPase Rac1, respectively. However, the upstream signals controlling Rac activity in this context have not yet been identified. By investigating the role of G proteins in PGC migration, we found that signaling mediated by G protein subunits Gβγ is required to regulate cell polarization. PGCs that are defective for Gβγ signaling failed to polarize, and developed multiple protrusions in random locations, resembling the defects observed in PGCs with decreased Rac activity. These defects render PGCs incapable of migrating actively and responding to directional cues. FRET-based assays showed that PGCs require Gβγ signaling for polarized Rac activation and actin organization at the leading front, as well as for maintaining overall Rac levels in these cells. Conversely, overexpression of Gβγ in PGCs increases Rac activity. Our results indicate that during PGC migration in vivo, Gβγ signaling regulates Rac activity to control cell polarity, which is required for the responsiveness to chemokine signaling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.